Phase contrast, inverted thiab pa microscopes - qhov sib txawv thiab zoo sib xws

Apr 18, 2024

Tso lus

Phase contrast, inverted thiab pa microscopes - qhov sib txawv thiab zoo sib xws

 

Cov no yog optical microscopes, siv lub teeb pom kev zoo raws li kev tshawb pom, tsis zoo li electron microscopes, scanning tunneling microscopes, atomic force microscopes, thiab lwm yam.
Tshwj xeeb:


Phase contrast microscopy, tseem hu ua Phase contrast microscopy. Qhov no yog vim lub teeb rays tsim ib theem me me sib txawv thaum lawv dhau los ntawm cov qauv pob tshab, thiab qhov sib txawv theem no tuaj yeem hloov pauv mus rau qhov kev hloov pauv loj lossis qhov sib piv ntawm cov duab kom nws tuaj yeem siv los ua duab. Nws tau tsim nyob rau xyoo 1930 los ntawm Fritz Zelnick hauv nws txoj kev tshawb fawb ntawm diffraction gratings. Rau qhov no nws tau txais txiaj ntsig Nobel nqi zog hauv Physics hauv xyoo 1953. Nws yog tam sim no dav siv los muab cov duab sib piv ntawm cov qauv pob tshab xws li cov hlwb nyob thiab cov ntaub so ntswg me.


Confocal Microscopy: Cov txheej txheem kho qhov muag uas siv qhov taw tes-los ntawm-taw tes illumination thiab spatial pinhole modulation kom tshem tawm cov teeb tawg ntawm lub dav hlau uas tsis yog focal ntawm tus qauv, tso cai rau kev txhim kho kho qhov muag thiab pom qhov sib txawv piv rau cov qauv duab. Kev sojntsuam lub teeb tawm los ntawm qhov chaw taw tes yog tsom los ntawm lub lens mus rau qhov khoom tau pom, thiab yog tias cov khoom yog raws nraim ntawm qhov focal point, lub teeb pom kev yuav tsum rov qab mus rau lub teeb ci los ntawm lub lens qub, uas yog hu ua confocal, los yog confocal luv luv. Confocal microscope nyob rau hauv lub teeb ntawm lub reflected lub teeb nyob rau hauv txoj kev nrog ib tug ib nrab-reflective ib nrab-lens (dichroic daim iav), yuav tau dhau los ntawm lub lens ntawm lub reflected lub teeb folded nyob rau hauv lwm yam kev taw qhia, nyob rau hauv lub tsom xam ntawm lub tsom xam nrog ib tug pinhole. (Pinhole), lub qhov yog nyob rau hauv lub focal point, lub baffle phaj qab lub photomultiplier raj (photomultiplier raj, PMT). Nws tuaj yeem xav txog tias lub teeb pom kev ua ntej thiab tom qab lub focal point ntawm lub teeb pom kev los ntawm cov txheej txheem confocal no, yuav tsis tuaj yeem tsom mus rau lub qhov me me, yuav raug thaiv los ntawm baffle. Yog li lub photometer ntsuas qhov siv ntawm lub teeb pom kev ntawm lub focal point. Qhov tseem ceeb ntawm qhov no yog tias cov khoom translucent tuaj yeem luam tawm hauv peb qhov ntev los ntawm kev txav lub lens system. Lub tswv yim zoo li no tau tawm tswv yim los ntawm Asmeskas kws tshawb fawb Marvin Minsky hauv xyoo 1953, thiab nws siv sijhawm 30 xyoo ntawm kev txhim kho los tsim lub tshuab tsom iav confocal uas tau ntsib Marvin Minsky cov tswv yim siv lub laser ua lub teeb ci.

 

3 Video Microscope -

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