Microscope Objective Parameters thiab cuam tshuam rau Micrograph Quality
Spherical Aberration
Ib tug monochromatic conical beam emissions los ntawm ib tug axial point ntawm lub kho qhov muag system hla lub tsheb ciav hlau kho qhov muag. Rays ntawm qhov sib txawv aperture tsis tuaj yeem sib sau ua ke ntawm tib lub ntsiab lus ntawm qhov chaw kho qhov muag, ua kom pom qhov muag plooj (feem ntau hu ua lub voj voog plooj) ntawm cov duab zoo tshaj plaws dav hlau perpendicular mus rau optical axis. Qhov kev ua yuam kev ntawm qhov kho qhov muag no hu ua spherical aberration.
Coma
Rau ib qho monochromatic conical beam emissions los ntawm ib tug off-axis khoom taw tes, tom qab refraction los ntawm lub kho qhov muag system, nws tsis muaj peev xwm tsim ib tug ntse taw tes nyob rau hauv lub zoo tagnrho duab dav hlau. Hloov chaw, nws tsim ib qho chaw zoo li lub hnub qub nrog lub ntsej muag ci. Qhov kev ua yuam kev no yog hu ua coma.
Astigmatism
Thaum lub oblique monochromatic conical beam los ntawm ib tug off-axis khoom taw tes mus los ntawm lub kho qhov muag system, nws ua tsis tau tejyam rau converge mus rau hauv ib tug ntse duab taw tes thiab tsuas yog ua rau qhov muag plooj. Qhov kev ua yuam kev no yog hu ua astigmatism.
Field Curvature
Cov duab ntse ntawm lub dav hlau tiaj tiaj perpendicular mus rau qhov kho qhov muag axis tsim los ntawm qhov kho qhov muag tsis yog dag ntawm lub dav hlau duab perpendicular mus rau qhov kho qhov muag axis, tab sis nyob rau ntawm ib qho nkhaus nto symmetrical txog qhov kho qhov muag axis, piv txwv li cov duab saum npoo yog nkhaus. Qhov no optical aberration yog teb curvature. Thaum tsom mus rau cov duab ntse ntawm qhov nruab nrab ntawm tus ncej, lub periphery ntawm daim duab yuav plooj; Thaum tsom mus rau cov duab ntse ntawm qhov chaw ib puag ncig, qhov nruab nrab ntawm daim duab tig plooj.
Chromatic Aberration
Thaum ib lub teeb ntawm lub teeb dawb los ntawm ib qho khoom dhau los ntawm qhov kho qhov muag, lub teeb ntawm qhov sib txawv wavelengths tsis tuaj yeem sib sau ua ke ntawm ib qho taw tes thiab tsim cov xim xim. Qhov no yog chromatic aberration. Nws tshwm sim vim hais tias lub refractive index ntawm tib lub iav qhov muag sib txawv rau lub teeb ntawm txawv wavelengths: lub refractive index yog siab dua rau luv wavelengths thiab qis dua rau ntev wavelengths.
Distortion
Ib txoj kab ncaj nraim nyob rau hauv cov khoom dav hlau uas dhau los ntawm qhov kho qhov muag axis zoo nkaus li nkhaus tom qab duab los ntawm qhov kho qhov muag. Qhov no imaging yuam kev yog distortion. Distortion tsuas yog cuam tshuam cov duab geometric ntawm cov duab thiab tsis txo cov duab sharpness. Qhov no yog qhov tseem ceeb ntawm qhov sib txawv ntawm qhov sib txawv ntawm qhov sib txawv thiab qhov sib txawv ntawm qhov sib txawv, coma, astigmatism thiab daim teb curvature.
Thaum ntsuam xyuas qhov zoo ntawm lub hom phiaj ntawm lub tshuab tsom iav, ntau qhov kev ua tau zoo feem ntau tau txais: kev daws teeb meem, qhov pom tseeb thiab qhov tob ntawm qhov chaw.
