Vim li cas electron microscopy qhov kev daws teeb meem siab dua li ntawm optical microscopy?
Qhov loj ntawm lub tshuab ntsuas qhov muag yog me dua li ntawm lub tshuab ntsuas hluav taws xob. Lub tshuab ntsuas qhov muag tsuas tuaj yeem saib xyuas cov qauv hauv microscopic xws li cov hlwb thiab chloroplasts, thaum lub tshuab hluav taws xob hluav taws xob tuaj yeem soj ntsuam cov qauv submicroscopic, xws li cov qauv ntawm organelles, kab mob, kab mob, thiab lwm yam.
Lub tshuab hluav taws xob tshuab hluav taws xob ua haujlwm ua kom nrawm thiab sib sau cov hluav taws xob sib txuas mus rau cov qauv nyias nyias, ua rau cov hluav taws xob sib tsoo nrog cov atoms hauv cov qauv thiab hloov cov kev taw qhia, ua rau lub tso suab angular scattering. Qhov luaj li cas ntawm lub kaum sab xis yog cuam tshuam nrog qhov ntom thiab tuab ntawm cov qauv, yog li nws tuaj yeem tsim cov duab nrog cov duab sib txawv. Cov duab yuav raug tso tawm rau ntawm cov khoom siv duab (xws li cov ntxaij vab tshaus fluorescent, cov yeeb yaj kiab, thiab cov khoom siv hluav taws xob sib txuas) tom qab kev nthuav dav thiab tsom.
Vim yog luv luv de Broglie wavelength ntawm electrons, qhov kev daws teeb meem ntawm kis tau tus mob electron microscopes ntau dua li ntawm optical microscopes, ncav cuag 0.1-0.2nm thiab magnification ntawm kaum tawm txhiab mus rau lab lub sij hawm. . Yog li ntawd, kev siv cov tshuab hluav taws xob xa hluav taws xob tuaj yeem siv los soj ntsuam cov qauv zoo ntawm cov qauv, thiab txawm tias saib cov qauv ntawm ib kab ntawm cov atoms, uas yog kaum tawm txhiab lub sij hawm me dua li cov qauv me tshaj plaws uas tuaj yeem pom nyob rau hauv ib qho. optical microscope. TEM yog ib qho tseem ceeb analytical txoj kev nyob rau hauv ntau yam scientific teb hais txog physics thiab biology, xws li kev tshawb fawb mob cancer, virology, ntaub ntawv science, raws li zoo raws li nanotechnology, semiconductor kev tshawb fawb, thiab hais txog.
