Lub hauv paus ntsiab lus ntawm fluorescence microscopy thiab laser confocal microscopy
fluorescence microscope
1. Lub tshuab fluorescence microscope yog ib qho khoom siv uas siv lub teeb ultraviolet los ua lub teeb ci kom pom cov khoom raug kuaj, ua rau nws tawm fluorescence, thiab tom qab ntawd saib cov duab thiab qhov chaw ntawm cov khoom hauv qab lub tshuab ntsuas. Fluorescence microscopy yog siv los kawm txog kev nqus, kev thauj mus los, kev faib tawm, thiab kev ua haujlwm hauv cheeb tsam ntawm cov tshuaj intracellular. Qee yam khoom hauv hlwb, xws li chlorophyll, tuaj yeem tawm fluorescence tom qab raug ultraviolet hluav taws xob; Txawm hais tias qee yam khoom lawv tus kheej tsis tuaj yeem tawm fluorescence, lawv tuaj yeem tawm fluorescence tom qab stained nrog fluorescent dyes lossis fluorescent antibodies thiab irradiated nrog ultraviolet lub teeb. Fluorescence microscopy yog ib qho ntawm cov cuab yeej rau kev tshawb fawb zoo thiab muaj nuj nqis ntawm cov tshuaj no.
2. Lub hauv paus ntsiab lus ntawm fluorescence microscope:
(A) Lub teeb qhov chaw: Lub teeb ci emits lub teeb ntawm ntau lub wavelengths (los ntawm ultraviolet mus rau infrared).
(B) Excitation filter light source: Transmitting light of a specific wavelength that can produce fluorescence in the specimen, while blocking light that is useless for excitation fluorescence.
(C) Fluorescent specimen: Feem ntau stained nrog fluorescent pigment.
(D) Blocking filter: xaiv kis fluorescence los ntawm kev thaiv cov excitation uas tsis tau nqus los ntawm cov qauv, thiab qee qhov wavelengths kuj tau xaiv hauv fluorescence. Lub microscope uas siv lub teeb ultraviolet ua lub teeb ci kom emit fluorescence los ntawm cov khoom irradiated. Lub tshuab ntsuas hluav taws xob tau tsim thawj zaug los ntawm Knorr thiab Harroska hauv Berlin, lub teb chaws Yelemees hauv xyoo 1931. Hom kab mob no siv cov kab hluav taws xob ceev ceev hloov cov kab teeb. Vim qhov luv luv wavelength ntawm cov electron ntws ntau dua piv rau lub teeb nthwv dej, qhov loj ntawm lub tshuab ntsuas hluav taws xob tuaj yeem ncav cuag 800000 zaug, nrog qhov kev daws teeb meem tsawg kawg ntawm 0.2 nanometers. Lub tshuab ntsuas hluav taws xob ntsuas hluav taws xob, uas tau pib siv nws hauv xyoo 1963, tso cai rau tib neeg pom cov qauv me me ntawm qhov chaw ntawm cov khoom.
3. Daim Ntawv Thov: Siv los nthuav cov duab ntawm cov khoom me me. Feem ntau siv rau kev soj ntsuam ntawm biology, tshuaj, microscopic hais, thiab lwm yam.
confocal microscope
1. Confocal microscope ntxiv ib lub teeb pom kev zoo ib nrab rau lub teeb pom kev, uas khoov lub teeb pom kev uas twb dhau los ntawm lub lens mus rau lwm qhov kev taw qhia. Muaj ib lub baffle nrog lub pinhole ntawm nws qhov chaw focal, thiab lub qhov me me nyob ntawm qhov chaw focal. Tom qab lub baffle yog ib lub raj photomultiplier. Nws tuaj yeem xav txog tias lub teeb pom kev ua ntej thiab tom qab pom lub teeb focal point tsis tuaj yeem tsom mus rau lub qhov me me los ntawm qhov system confocal, thiab yuav raug thaiv los ntawm baffle. Yog li dab tsi lub photometer ntsuas yog qhov kev siv ntawm lub teeb pom kev ntawm lub focal point.
2. Lub hauv paus ntsiab lus: Cov khoom siv kho qhov muag ib txwm siv lub teeb pom kev hauv thaj chaw, thiab cov duab ntawm txhua lub ntsiab lus ntawm cov qauv raug cuam tshuam los ntawm qhov sib txawv lossis tawg tawg ntawm cov ntsiab lus uas nyob ib sab; Lub laser scanning confocal microscope siv lub teeb pom kev zoo tsim los ntawm lub teeb ci laser hla los ntawm lub teeb pom kev los ntsuas txhua qhov taw qhia hauv lub dav hlau focal ntawm cov qauv. Lub teeb pom kev zoo ntawm cov qauv yog duab ntawm qhov kev sojntsuam pinhole, thiab tau txais taw tes los ntawm taw tes lossis kab los ntawm photomultiplier raj (PMT) lossis thermoelectric coupling ntaus ntawv (cCCD) tom qab kev sojntsuam pinhole, sai sai tsim cov duab fluorescent ntawm lub computer saib lub vijtsam . Lub illumination pinhole thiab nrhiav pom pinhole yog conjugated txheeb ze rau lub dav hlau focal ntawm lub hom phiaj lens. Cov ntsiab lus ntawm lub dav hlau focal yog ib txhij tsom rau ntawm lub teeb pom kev zoo thiab lub emission pinhole, thiab cov ntsiab lus sab nraud ntawm lub dav hlau focal tsis yog duab ntawm qhov pom pinhole. Qhov no ua rau cov duab confocal uas yog qhov kho qhov muag hla ntu ntawm cov qauv, kov yeej qhov tsis zoo ntawm qhov muag plooj hauv cov duab tsom iav dav dav.
3. Daim ntawv thov teb: koom nrog tshuaj, tsiaj txhu thiab nroj tsuag kev tshawb fawb, biochemistry, bacteriology, cell biology, nqaij embryology, zaub mov science, noob caj noob ces, pharmacology, physiology, optics, pathology, botany, neuroscience, marine biology, ntaub ntawv science, electronics science, mechanics , petroleum geology, thiab mineralogy.
