Qhov txawv ntawm Confocal Laser Microscopy thiab Fluorescence Microscopy
1. Cov ntsiab cai sib txawv
1. Fluorescence microscope: Hom microscope no ua rau siv lub teeb ultraviolet los irradiate cov khoom raug kuaj xyuas kom nws tawm fluorescence. Tom qab no, daim ntawv thiab qhov chaw ntawm cov khoom raug soj ntsuam.
2. Laser confocal microscope: fluorescent probes yog qhib los ntawm ultraviolet los yog pom lub teeb, thiab ib tug laser scanning ntaus ntawv yog muab tso rau lub hauv paus ntawm fluorescence microscopy imaging.
Ob, yam ntxwv sib txawv
1. Fluorescence microscope: siv los tshuaj xyuas seb cov tebchaw puas absorbed, thauj, faib, thiab nyob hauv cov hlwb, thiab lwm yam. Txawm hais tias qee qhov sib txuas tsis tuaj yeem ci ntawm lawv tus kheej, lawv tuaj yeem fluoresce thaum stained nrog fluorescent dyes lossis fluorescent antibodies tom qab raug UV teeb, ib yam li cov ntaub ntawv rau qee cov molecules pom hauv hlwb, xws li chlorophyll.
2. Kev ua haujlwm ntawm laser confocal microscope tso cai rau kev soj ntsuam ntawm lub cev muaj zog xws li Ca2 ntxiv , pH, membrane muaj peev xwm, thiab kev hloov pauv hauv cell morphology ntawm qib subcellular, nrog rau cov duab fluorescent ntawm cov qauv me me hauv cov hlwb lossis cov ntaub so ntswg.
Peb, kev siv sib txawv
1. Fluorescence microscopy: Cov cuab yeej tseem ceeb hauv immunofluorescence cytochemistry yog fluorescence microscope. Nws yog tsim los ntawm cov khoom tseem ceeb xws li optical system, lim phaj system, thiab lub teeb ci. Lub hom phiaj yog txhawm rau txhawb nqa cov qauv kom tawm fluorescence siv ib lub teeb ntawm lub teeb, thiab tom qab ntawd saib cov duab fluorescence ntawm cov qauv siv lub hom phiaj loj ntawm lub lens thiab lub qhov muag.
2. Laser confocal microscopy: Thaum ua ke nrog lwm cov thev naus laus zis zoo sib xws, laser scanning confocal microscopy tau siv dav hauv thaj chaw ntawm molecular cell biology xws li morphology, physiology, thiab immunology. Nws muaj cov kev tshawb fawb siv tau xws li kev ntsuas fluorescence ntau thiab ntsuas cov duab ntau.
