Daim ntawv thov txheej txheem ntawm pH meter hauv kev kuaj cov khoom siv mis nyuj
Yuav ua li cas ntxuav cov khoom siv mis nyuj tom qab siv lub ntsuas acidity, thiab yuav ua li cas ntxuav cov khoom noj muaj protein ntau thiab cov khoom noj muaj protein ntau. Thaum ntsuas cov xov xwm sib txawv lossis ua haujlwm nyob rau hauv ib puag ncig sib txawv, qhov xwm txheej ntawm cov kab mob electrode sib txawv, yog li cov txheej txheem ntxuav yuav raug tshuaj xyuas kom meej. Muaj ntau ntau hom kev tu rau pH ntsuas. Ceev faj thaum lub sij hawm txheej txheem, raws li iav pH electrodes feem ntau yog siv los ntsuas cov tshuaj no. Ntxuav cov sediment sab laug ntawm lub electrode:
Thaum kuaj pom cov mis nyuj muaj protein ntau:
Soak hauv hydrochloric acid tov nrog 1 gastric protease (HCl concentration ntawm 0.1mol/L) rau 15 feeb, los yog tsau nyob rau hauv 10% sodium hypochlorite tov rau 5 feeb. Tom qab ntawd, ntxuav nrog dej ntshiab thiab tsau rau hauv 3.3mol / L KCL electrode soaking tov rau 12 teev kom muaj peev xwm sib npaug ntawm electrode. Qhov no tuaj yeem rov kho nws cov haujlwm qub, yog tias
Soak cov electrodes hauv cov tshuaj ntxuav tais diav rau 1 teev. Tshem tawm cov electrode thiab yaug kom huv si, tom qab ntawd tsau nws hauv cov tshuaj gastric protease rau 4 teev. Txoj kev npaj rau gastric protease tov: Sib tov 5% gastric protease 0.5MOL / L hydrochloric acid tov nyob rau hauv ib tug ratio ntawm 1: 1 * Tom qab ntawd, tshem tawm cov electrode, ntxuav nws nrog distilled dej, thiab tsau nyob rau hauv ib tug 3mol. /L (yog 3.3mol) KCL tov rau tom qab siv. Cov electrode yuav tsum tau ntim nrog 3MOL / L KCL tov kom tsis txhob qhuav
Cov khoom noj mis nyuj muaj cov yam ntxwv ntawm cov protein ntau thiab cov rog, uas tuaj yeem yooj yim ua rau muaj kev cuam tshuam ntawm electrode iav pob capillary. Ib qho electrode nyob rau hauv ib txwm siv yuav muaj kev teb qeeb thiab kev nyeem tsis ruaj khov tom qab peb lub hlis; Txhawm rau txuag cov nqi siv thiab faib cov peev txheej tsim nyog.
Vim tias acidity ntsuas tau dav siv hauv kev lag luam mis nyuj. Tshwj xeeb tshaj yog nyob rau hauv kev ntsuam xyuas ntawm ib nrab thiab tiav cov khoom, nws plays lub luag hauj lwm tseem ceeb heev, thiab xws li pH qhov tseem ceeb qhia txog qhov zoo ntawm cov tshuaj ntsuam xyuas. Yog tias tus nqi pH tsis tuaj yeem ntsuas tau raws sijhawm rau kev siv khoom siv, nws yuav muaj kev cuam tshuam tsis zoo rau kev tsim khoom.
