MERGE txoj kev rau fluorescence microscope duab
Peb feem ntau pom cov duab fluorescent nthuav tawm nyob rau hauv cov khoom ua ke, raws li qhia hauv qab no. Los ntawm kev sib koom ua ke, koj tuaj yeem paub qhov txawv ntawm ob (lossis ntau dua) cov teeb liab fluorescent ntawm tib qhov chaw hauv cov ntaub so ntswg lossis cell sib tshooj lossis tsis. Niaj hnub no, cia peb tham txog yuav ua li cas ua ke fluorescence microscope duab.
1, Lub hauv paus ntsiab lus
Lub hauv paus ntsiab lus tseem ceeb yog rov xam cov ntaub ntawv xim ntawm tib lub pixel hauv ob (lossis ntau dua) cov duab sib npaug raws li cov qauv kom tau txais xim tshiab. Piv txwv li, liab thiab ntsuab ntawm ib daim duab fluorescent sib tshooj los ua daj. Qhov no algorithm yog raws li RGB "ntxiv xim hom" (raws li qhia hauv qab no), thiab Photoshop txheej fusion hom "xim dropout" yuav luag tib yam.
2, software qhia
Niaj hnub no peb qhia txog kev tshawb fawb txog kev tshawb fawb hauv cov software xws li: ImageJ, nrog rau kev sib koom ua ke cov duab dua li nrog Ps yooj yim dua, ncaj qha ntau dua (tsis quav ntsej txog txheej, txheej txheej).ImageJ tsis tas yuav tsum tau teeb tsa, software tuaj yeem txawm tias qhib rau ntawm USB lo siv.
Muaj ob txoj hauv kev los sib koom ua ke cov duab fluorescence, ib qho yog ua ke nrog cov software uas tuaj nrog lub tshuab tsom, thiab lwm qhov yog khaws cov duab fluorescence ntawm tib qhov chaw saib nrog cov teeb pom kev sib txawv, thiab tom qab ntawd muab lawv sib xyaw nrog cov tsis tu ncua. Cov duab ua software (xws li PS) thaum nthuav tawm cov txiaj ntsig.Yog tias koj txhawj xeeb txog kev tua cov fluorescence thaum saib cov fluorescence microscope (tshwj xeeb tshaj yog thaum stained los ntawm fluorescent dyes), koj tuaj yeem nqa ib pawg ntawm cov duab sai sai, thiab tom qab ntawd xaiv cov. uas yog qhov zoo thaum rov qab los. Thaum saib ntawm fluorescence microscope, yog tias koj txhawj xeeb txog fluorescence quenching (tshwj xeeb tshaj yog nrog cov fluorescent dye staining), koj tuaj yeem ceev nrooj snap ib pawg duab ua ntej, thiab tom qab ntawd rov qab mus ua ke cov uas zoo rau kev nthuav tawm cov txiaj ntsig. Hauv qhov no, txoj kev thib ob yuav hloov tau yooj yim dua thiab xis nyob.
