Laser confocal microscope laser siv kev hloov kho
Muaj ob qhov kev hloov kho rau laser siv: lub hwj chim tswj pob qhov rooj thiab Acousto-Optical Tunable Filter (AOTF).
1. lub hwj chim tswj pob qhov rooj: feem ntau teem rau 30% ntawm tus nqi loj, qhov tso zis ntau ntxiv, lub neej ntawm laser yuav luv luv, tab sis kuj ua rau lub suab nrov laser, yuav tsum tau kho thawj AOTF, PMT, thiab lwm yam kom tau txais qhov zoo tshaj plaws siv.
2. Acousto-optic tunable lim: tswj lub excitation beam thiab siv, tuaj yeem qhib lossis kaw lub laser hauv milliseconds lossis hloov pauv ntawm 0-100%. Qhov no ua rau nws muaj peev xwm siv lub laser sib txawv rau txhua qhov duab taw tes thiab xaiv qhov xav tau siv.
Lub hauv paus ntsiab lus ntawm kev ua haujlwm: Bragg diffraction nyhuv ntawm lub suab nthwv dej propagating nyob rau hauv ntau yam xov xwm ntawm qhov tshwm sim mus rau hauv lub propagation nruab nrab yog siv. Thaum lub tswv yim ntawm qee zaus ntawm cov xov tooj cua zaus, AOTF diffraction ntawm qhov teeb meem polychromatic teeb, los ntawm qhov uas lub wavelength raug xaiv rau hauv lub teeb monochromatic, monochromatic wavelength thiab xov tooj cua zaus f muaj ib qho kev sib txuas lus ib-rau-ib, ntev npaum li cov hluav taws xob. teeb liab tuning tuaj yeem ua tau sai thiab randomly hloov lub wavelength ntawm cov zis tawm. AOTF kom ua tiav qhov kev hloov kho tas li ntawm lub laser siv, thiab muaj kev hloov kho sai, kom ua tiav qhov kev txhawb nqa hauv zos thiab kev soj ntsuam, hloov lub wavelength sai (hauv laser confocal microscopy, photomultiplier tsis nce qhov kev siv ntawm lub laser, tab sis tswj qhov siv. ntawm photoelectricity uas yog imaged, yog li qhov ci ntawm cov duab tuaj yeem kho los ntawm kev kho qhov voltage ntawm photomultiplier Tsis tas li ntawd, qhov loj ntawm lub confocal pinhole cuam tshuam rau tus nqi ntawm photons, uas kuj kho qhov ci ntawm daim duab photomultiplier yog lub tshuab nqus tsev tshwj xeeb uas tsis tshua muaj siab rau ultraviolet, pom, thiab ze-infrared teeb, thiab tuaj yeem txhim kho lub teeb pom kev tsis muaj zog tuaj txog li 108 npaug ntawm nws tus nqi qub kom ntsuas lub teeb liab.
