Yuav ua li cas kho lub laser siv ntawm Confocal Microscope
Laser siv muaj ob yam kev hloov kho: lub hwj chim tswj pob qhov rooj, acousto-optic tunable lim
1. Fais fab tswj pob qhov rooj: feem ntau teem rau 30 feem pua ntawm tus nqi siab tshaj plaws. Yog tias qhov tso zis ntau ntxiv, lub neej ntawm lub laser yuav luv luv, thiab lub suab nrov laser kuj yuav raug tsim. AOTF, PMT, thiab lwm yam yuav tsum tau kho ua ntej kom tau txais qhov zoo tshaj plaws siv.
2. Acousto-optic tunable lim: tswj lub excitation teeb beam thiab siv, qhib los yog tawm lub laser los yog kho kom hloov ntawm 0-100 feem pua hauv milliseconds. Qhov no ua rau nws muaj peev xwm siv lub laser sib txawv rau txhua qhov duab taw tes thiab xaiv qhov xav tau siv.
Txoj hauv kev ua haujlwm: Nws siv Bragg diffraction nyhuv ntawm lub suab nthwv dej tshwm sim ntawm qhov nruab nrab ntawm kev nthuav tawm thaum nws nthuav tawm hauv ntau yam xov xwm. Thaum lub xov tooj cua zaus teeb liab ntawm qee zaus yog cov tswv yim, AOTF cuam tshuam qhov teeb meem polychromatic teeb, thiab xaiv lub teeb monochromatic nrog lub wavelength ntawm λ. Lub wavelength ntawm qhov tso zis hloov pauv tuaj yeem hloov tau sai thiab randomly. AOTF paub txog kev hloov kho tas li ntawm laser siv, thiab muaj kev hloov kho sai, paub txog kev txhawb nqa hauv zos thiab kev soj ntsuam, thiab kev hloov ceev wavelength (microsecond theem). Yog li ntawd, qhov ci ntawm cov duab tuaj yeem hloov kho los ntawm kev kho qhov voltage ntawm photomultiplier raj. Tsis tas li ntawd, qhov luaj li cas ntawm tus pinhole (confocal pinhole) cuam tshuam rau tus nqi ntawm photons thiab tuaj yeem kho qhov ci ntawm daim duab. Lub raj photomultiplier yog lub tshuab nqus tsev tshwj xeeb uas tsis tshua muaj siab rau ultraviolet lub teeb, pom lub teeb thiab ze-infrared teeb. Nws tuaj yeem ua rau cov teeb liab tsis muaj zog tuaj mus rau 108 npaug ntawm qhov qub, kom lub teeb pom kev zoo tuaj yeem ntsuas.
