Yuav ua li cas Electron Microscopy Samples Npaj
1. Txoj kev kwv yees
Nyob rau hauv lub tshuab nqus tsev, qhov saum npoo ntawm cov qauv yog txau nrog cov hlau hnyav atoms nrog lub zog hluav taws xob tawg, kom qhov sib txawv muaj zog yog tsim los ntawm cov qauv thiab qhov chaw tsis muaj tshuaj tsuag, thiab qhov tsis tau txau yuav ua rau qhov projection ntawm tus qauv. Raws li qhov projection, peb Cov duab peb sab thiab qhov siab ntawm cov qauv tuaj yeem nkag siab, thiab cov qauv no feem ntau yog siv los soj ntsuam cov flagella ntawm cov kab mob lossis cov kab mob ntawm cov kab mob.
2. Txoj kev tsis zoo staining
Vim hais tias txoj kev no stains keeb kwm yav dhau ntawm cov qauv los qhia cov qauv, nws yog hu ua tsis zoo staining. Feem ntau, cov qauv yog ib puag ncig los ntawm cov khoom uas muaj cov khoom siv hluav taws xob siab, tsis pom cov qauv nws tus kheej, thiab tsis hnov mob nrog cov qauv, xws li sodium ntsev ntawm phosphotungstic acid. Cov duab kuj tuaj yeem cuam tshuam cov qauv sab hauv ntawm cov qauv mus rau qee yam. Ua li no, cov kab mob hlwb, kab mob, thiab lwm yam tuaj yeem pom.
3. Ultrathin seem txoj kev
Qhov no yog txoj kev siv ntau tshaj plaws vim tias nws tso cai rau kev soj ntsuam cov qauv zoo hauv cov hlwb lossis lwm cov qauv. Feem ntau nws yog tsim nyog los kho cov qauv raws li ib tug tej yam txheej txheem, dehydrate nws, ces embed nws nyob rau hauv resin raws li kev txhawb nqa, thiab txiav nws mus rau hauv nyias slices nrog ib tug ultra-nyias microtome. Vim tias tsuas yog cov hlais nrog lub thickness ntawm 20 txog 100 nanometers tuaj yeem pom nrog lub tshuab hluav taws xob xa hluav taws xob, hauv cov qauv kab mob dav dav, ib lub xov tooj yuav tsum muab faib ua 10 mus rau 50 daim.
Txhawm rau xaiv lub tshuab ntsuas hluav taws xob, ua ntej ntawm tag nrho, xyuas seb lub chaw tsim khoom siv hluav taws xob siv hluav taws xob puas muaj daim ntawv pov thawj tiav; thib ob, saib daim ntawv cog lus ntawm lub chaw tsim khoom siv hluav taws xob tshuab hluav taws xob; thib ob, nkag siab txog cov kev pabcuam ntawm cov chaw tsim khoom siv hluav taws xob tshuab hluav taws xob; Tsis tas li ntawd, saib cov txheej txheem tsis zoo ntawm lub tshuab ntsuas hluav taws xob scanning.
Ntxiv nrog rau kev xaiv cov tshuab luam ntawv electron microscope cov tuam txhab, thaum yuav cov khoom siv hluav taws xob sib txuas, nws tseem yuav tsum xav txog qhov ua tau zoo thiab cov kev qhia ntawm lub tshuab ntsuas hluav taws xob, seb nws puas tuaj yeem ua tau raws li peb cov kev xav tau niaj hnub tsom xam, thiab tseem yuav tsum xav txog. ntau yam thaum yuav khoom. Tsuas yog nyob rau hauv txoj kev no peb tuaj yeem ua tau raws li peb qhov kev sim thiab kev tshuaj xyuas.
