Fluorescence microscopes tuaj yeem muab faib ua ob hom raws li txoj cai ntawm txoj kev teeb:
1. Transmission fluorescence microscope
Nyob rau hauv cov laus fluorescence microscopes, lub excitation lub teeb ci dhau los ntawm cov khoom siv los ntawm lub condenser kom excite fluorescence. Qhov kom zoo dua yog tias cov fluorescence muaj zog ntawm qhov tsis tshua muaj magnification, tab sis qhov tsis zoo yog tias cov fluorescence tsis muaj zog thaum lub magnification nce. Yog li nws tsuas yog tsim nyog rau kev soj ntsuam cov ntaub ntawv loj dua.
2.Epifluorescence microscopy
Lub teeb excitation ntog downward los ntawm lub hom phiaj lens mus rau qhov chaw kuaj, piv txwv li, tib lub hom phiaj lens yog siv raws li lub teeb pom kev zoo condenser thiab lub hom phiaj lens rau sau fluorescence.
Lub dichroic beam splitter (dichroic daim iav) yuav tsum tau muab ntxiv rau txoj kev kho qhov muag, uas yog nyob rau ntawm lub kaum sab xis ntawm 45o rau qhov kho qhov muag axis. Lub teeb excitation yog reflected rau hauv lub hom phiaj lens thiab concentrated ntawm tus qauv. Cov fluorescence tsim los ntawm cov qauv yog cuam tshuam los ntawm qhov chaw ntawm lub hom phiaj lens thiab lub hau. Lub teeb pom kev zoo tshwm sim nyob rau saum npoo ntawm lub iav swb nkag mus rau lub hom phiaj lens tib lub sijhawm thiab rov qab mus rau lub dichroic beam splitter kom cais cov excitation teeb thiab fluorescence. Qhov seem excitation teeb yog ces absorbed los ntawm cov lim thaiv. Yog tias koj siv cov lim dej sib txawv / ob-xim beam splitter / thaiv cov lim ua ke, koj tuaj yeem ua tau raws li qhov xav tau ntawm cov khoom siv tshuaj tiv thaiv fluorescence sib txawv.
Qhov kom zoo dua ntawm hom fluorescence microscope no yog qhov kev pom zoo sib xws, cov duab pom tseeb, thiab qhov loj dua qhov loj dua, qhov muaj zog fluorescence.
Dab tsi yog pawg tseem ceeb ntawm optical microscopes?
1. Ordinary optical microscope
Ordinary optical microscopes feem ntau yog tsim los ntawm cov hauv qab no: lub teeb pom kev zoo, uas suav nrog lub teeb ci thiab condenser; optical magnification system, uas yog tsim los ntawm lub hom phiaj lens thiab eyepiece. Nws yog qhov tseem ceeb tshaj plaws ntawm lub tshuab tsom iav thiab yog tsim los kom tsis txhob muaj ntau qhov sib txawv ntawm lub ntsej muag thiab chromatic aberration, ob lub qhov muag thiab lub hom phiaj lens yog tsim los ntawm cov pab pawg lens complex.
2. Laser confocal scanning microscope
Lub laser confocal scanning microscope suab nrov heev thiab nyuaj. Qhov tseeb, nws tsuas yog siv laser ua lub teeb ci scanning kom sai sai thiab cov duab khoom taw tes ntawm taw tes, kab los ntawm kab, thiab ntog los ntawm qhov chaw.
Raws li qhov luv luv wavelength ntawm lub laser beam, lub beam nws tus kheej yog nyias nyias, uas txiav txim siab tias confocal laser scanning microscope muaj ib tug siab daws teeb meem, uas yog hais txog peb npaug ntawm ib tug dog dig optical microscope. Hom microscope no yog siv los soj ntsuam cell morphology thiab quantitatively analyze intracellular biochemical Cheebtsam thiab ntsuas cell morphology.
3. Tsaus teb microscope
Muaj ib lub teeb phaj nyob rau hauv nruab nrab ntawm lub condenser ntawm ib tug maub-field microscope, uas tiv thaiv illumination lub teeb los ntawm nkag mus rau lub hom phiaj lens ncaj qha. Tsuas yog lub teeb pom kev thiab cuam tshuam los ntawm cov qauv tuaj yeem nkag mus rau lub hom phiaj lens. Yog li ntawd, lub keeb kwm yav dhau ntawm qhov chaw saib yog dub thiab cov npoo ntawm cov khoom yog ci.
