Fluorescence microscopes tuaj yeem muab faib ua ob hom raws li lawv txoj kev kho qhov muag:
1. Transmission fluorescence microscope: Lub teeb pom kev zoo yog siv los ua kom muaj fluorescence los ntawm kev hla cov khoom siv los ntawm lub lens condenser. Feem ntau siv cov xim tsaus nti concentrators tuaj yeem siv tau, thiab cov twj tso kua mis zoo tib yam tuaj yeem siv los kho cov reflector rau redirect thiab sidelobe lub teeb excitation rau cov qauv. Qhov no yog qhov qub-fashioned fluorescence microscope. Nws qhov zoo dua yog qhov muaj zog fluorescence ntawm qhov kev nthuav dav tsawg, tab sis nws qhov tsis zoo yog tias nws cov fluorescence txo qis nrog kev nthuav dav ntxiv. Yog li ntawd, nws yog qhov zoo dua rau kev soj ntsuam cov ntaub ntawv loj dua.
2. Poob lub teeb fluorescence microscope yog ib hom tshiab ntawm fluorescence microscope tsim nyob rau niaj hnub no. Tsis zoo li cov saum toj no, lub teeb excitation poob los ntawm lub hom phiaj lens downwards mus rau saum npoo ntawm cov qauv, siv tib lub hom phiaj lens raws li lub illumination condenser thiab lub hom phiaj lens rau sau fluorescence. Ib ob lub xim beam splitter yuav tsum tau ntxiv rau txoj kev kho qhov muag, uas yog 45 degrees sib nrug ntawm lub teeb uranium. Lub kaum sab xis, lub teeb excitation yog reflected rau hauv lub hom phiaj lens thiab tsom rau cov qauv. Cov fluorescence tsim los ntawm cov qauv, nrog rau lub teeb pom kev zoo tshwm sim los ntawm qhov chaw ntawm lub hom phiaj lens thiab npog iav, nkag mus rau lub hom phiaj lo ntsiab muag thiab rov qab mus rau ob lub xim beam splitter, sib cais lub teeb excitation thiab fluorescence. Lub teeb excitation residual yog absorbed los ntawm cov lim thaiv. Yog tias sib xyaw ua ke ntawm excitation lim / dual xim beam separators / thaiv cov ntxaij lim dej siv, lawv tuaj yeem ua tau raws li qhov xav tau ntawm cov khoom sib txawv fluorescent. Qhov zoo ntawm no fluorescence microscope yog cov teeb pom kev zoo hauv cheeb tsam, pom meej meej, thiab muaj zog fluorescence nrog qhov loj dua.
Cov lus qhia rau kev siv fluorescence microscope
1. Qhib lub teeb ci. Lub teeb ultra-siab mercury yuav tsum tau preheated rau ob peb feeb kom ncav cuag nws qhov siab tshaj plaws ci.
2. Kev xa tawm fluorescence microscopy yuav tsum tau teeb tsa ntawm qhov xav tau excitation lim ntawm lub teeb qhov chaw thiab lub condenser,
thiab qhov sib thooj thaiv lim qab lub hom phiaj lens. Lub teeb poob fluorescence microscope yuav tsum tau ntxig rau qhov yuav tsum tau lim excitation / dual xim beam splitter / thaiv lim thaiv rau hauv lub qhov ntawm txoj kev kho qhov muag.
3. Saib xyuas nrog lub tshuab ntsuas hluav taws xob qis thiab kho qhov chaw nruab nrab ntawm lub teeb pom kev zoo raws li cov cuab yeej hloov kho ntawm ntau hom fluorescence microscopes, kom nws nyob ntawm qhov chaw ntawm tag nrho cov teeb pom kev zoo.
4. Muab cov qauv thiab tsom rau kev soj ntsuam. Kev ceev faj yuav tsum tau them thaum siv: Tsis txhob saib ncaj qha nrog koj ob lub qhov muag ua ntej txhim kho lub lim kom tsis txhob muaj qhov muag puas; Thaum soj ntsuam cov qauv nrog lub tshuab ntsuas roj, yuav tsum siv lub tshuab ntsuas roj tshwj xeeb uas tsis muaj fluorescent; Tom qab lub teeb hluav taws xob siab mercury raug kaw, nws tsis tuaj yeem tig rov qab tam sim ntawd. Nws yuav siv sij hawm 5 feeb los pib dua, txwv tsis pub nws yuav tsis ruaj khov thiab cuam tshuam rau lub neej ntawm lub teeb mercury.
Kev soj ntsuam nyob rau hauv lub fluorescence microscope ntawm ib qho kev qhia platform siv lub teeb xiav violet lim, hlwb stained nrog 0.01% acridine txiv kab ntxwv fluorescent dye tuaj yeem pom. Lub nucleus thiab cytoplasm zoo siab los tsim ob lub xim sib txawv ntawm fluorescence (ntsuab ntsuab thiab txiv kab ntxwv liab).
