Fluorescence microscope specimen npaj yuav tsum
(i) Slides
Lub thickness ntawm tus swb yuav tsum nyob nruab nrab ntawm 0.8 thiab 1.2 hli, tuab heev slopes, ntawm ib sab tes, ntau lub teeb haum, ntawm qhov tod tes, tsis tuaj yeem ua rau lub zog excitation ntawm lub teeb nyob rau hauv cov qauv sau. Cov swb yuav tsum du, tuab tuab, tsis muaj qhov pom tseeb autofluorescence. Qee zaum yuav tsum tau siv quartz iav slides.
(ii) daim ntaub
Cov ntawv npog yog li ntawm 0.17mm tuab thiab du. Txhawm rau txhim kho lub teeb pom kev zoo, kuj tuaj yeem siv los cuam tshuam nrog lub npog npog, qhov no yog ib qho tshwj xeeb nto plated nrog ntau txheej ntawm cov wavelengths sib txawv ntawm lub teeb ua lub luag haujlwm sib txawv hauv kev cuam tshuam ntawm cov khoom (xws li magnesium fluoride) ntawm cov npog npog, uas tuaj yeem ua rau cov fluorescence los ntawm tus du thiab muaj kev cuam tshuam qhov excitation ntawm lub teeb, lub reflection ntawm excitation ntawm lub teeb poj niam yuav excitation ntawm cov qauv.
(C) cov qauv
Cov ntaub so ntswg los yog lwm cov qauv tsis tuaj yeem tuab heev, xws li tuab heev excitation teeb feem ntau yog siv rau hauv qis ntawm cov qauv, thiab lub hom phiaj lens ncaj qha pom nyob rau sab sauv ntawm qhov tsis txaus excitation. Tsis tas li ntawd, cell overlap los yog impurities npog, cuam tshuam rau kev txiav txim.
(D) sealing agent
Sealing tus neeg sawv cev feem ntau siv glycerol, yuav tsum tsis muaj autofluorescence, tsis muaj xim thiab pob tshab, qhov ci ntawm fluorescence hauv pH 8.5 ~ 9.5 thaum ci dua, tsis yooj yim kom ploj mus sai. Yog li ntawd, feem ntau siv glycerin thiab 0.5 mol / l pH9.0 ~ 9.5 ntawm cov carbonate tsis sib npaug ntawm cov sib tov rau cov sealant.
(E) iav roj
General darkfield fluorescence microscope thiab roj tsom iav tsom iav, yuav tsum siv daim iav roj, * zoo siv cov roj tshwj xeeb uas tsis muaj fluorescent daim iav, kuj tseem siv tau rau qhov chaw saum toj no glycerol, kua paraffin kuj siv tau, tab sis qhov refractive index qis dua. , cov duab zoo yog me ntsis cuam tshuam.
