Qhov sib txawv ntawm qhov zoo tib yam optical microscope thiab fluorescence-based microscope
Optical microscope: Nws yog ib qho cuab yeej kho qhov muag uas siv cov qauv kho qhov muag kom nthuav dav thiab duab cov khoom me me uas tsis tuaj yeem txawv ntawm tib neeg lub qhov muag, kom tib neeg tuaj yeem muab cov ntaub ntawv ntxaws ntxaws.
Fluorescence microscope: Lub fluorescence microscope siv lub teeb ci ultraviolet ua lub teeb ci los teeb pom cov khoom raug tshuaj xyuas kom nws tawm fluorescence, thiab tom qab ntawd saib cov duab thiab qhov chaw ntawm cov khoom hauv qab lub tshuab tsom. Fluorescence microscopy yog siv los kawm txog kev nqus thiab thauj cov khoom hauv cov hlwb, nrog rau kev faib tawm thiab qhov chaw ntawm cov tshuaj lom neeg. Qee yam khoom hauv hlwb, xws li chlorophyll, tuaj yeem fluoresce tom qab irradiated los ntawm ultraviolet rays; Qee cov khoom lawv tus kheej tsis tuaj yeem fluoresce, tab sis yog tias lawv stained nrog fluorescent dyes lossis fluorescent antibodies, lawv tuaj yeem fluoresce tom qab irradiation nrog ultraviolet rays. Fluorescence microscopy yog ib qho ntawm cov cuab yeej rau kev tshawb fawb zoo thiab ntau yam ntawm cov khoom no.
Optical microscopes feem ntau muaj ib theem, lub teeb tsom teeb pom kev zoo, lub hom phiaj lens, lub ntsej muag thiab lub ntsej muag tsom. Los ntawm kev hloov txoj kev teeb pom kev zoo, lub teeb pom kev zoo teeb pom kev zoo tuaj yeem tau txais cov kev soj ntsuam sib txawv xws li cov ntsiab lus tsaus nti ntawm qhov ci ci tom qab (hu ua lub teeb pom kev zoo) lossis cov ntsiab lus ci ntawm qhov tsaus ntuj (hu ua tsaus ntuj teeb pom kev zoo), kom nws tuaj yeem ua tau. siv nyob rau hauv cov xwm txheej sib txawv. kom zoo dua nrhiav thiab saib cov qauv zoo.
Qhov sib txawv ntawm fluorescence microscope thiab dog dig microscope yog raws li nram no:
1. Txoj kev teeb pom kev zoo feem ntau yog epi-illumination, uas yog, lub teeb qhov chaw yog projected ntawm tus qauv los ntawm lub hom phiaj lens;
2. Lub teeb ci yog ultraviolet lub teeb, nrog luv luv wavelength thiab siab dua qhov kev daws teeb meem zoo tib yam microscopes;
3. Muaj ob lub lim dej tshwj xeeb, ib qho nyob rau hauv pem hauv ntej ntawm lub teeb ci yog siv los lim pom lub teeb, thiab ib qho nruab nrab ntawm lub qhov muag thiab lub hom phiaj lens yog siv los lim ultraviolet rays los tiv thaiv tib neeg lub qhov muag.
Fluorescence microscopy kuj yog hom optical microscope. Qhov sib txawv tseem ceeb yog qhov sib txawv excitation wavelengths. Qhov no txiav txim siab qhov sib txawv ntawm cov qauv thiab kev siv ntawm fluorescence microscopes thiab dog dig optical microscopes.
Fluorescence microscopy yog ib qho cuab yeej tseem ceeb hauv immunofluorescence cytochemistry. Nws yog tsim los ntawm cov khoom tseem ceeb xws li lub teeb ci, lim phaj system thiab optical system. Nws siv lub teeb ntawm qee qhov wavelength los ua kom lub cev muaj zog kom tawm fluorescence, thiab tom qab ntawd nthuav tawm cov duab fluorescence ntawm cov qauv los ntawm lub hom phiaj lens thiab eyepiece system.
