Qhov txawv ntawm Ordinary Light Microscope thiab Fluorescence Microscope
Optical microscope: Nws yog ib qho cuab yeej kho qhov muag uas siv cov qauv kho qhov muag kom nthuav dav thiab duab cov khoom me me uas tsis tuaj yeem daws tau los ntawm tib neeg lub qhov muag, kom tib neeg tuaj yeem rho tawm cov ntaub ntawv microstructure.
Fluorescence microscope: Fluorescence microscope siv lub teeb ci ultraviolet ua lub teeb ci rau irradiate cov khoom hauv kev tshuaj xyuas kom nws tawm fluorescence, thiab tom qab ntawd saib cov duab thiab qhov chaw ntawm cov khoom hauv qab lub tshuab tsom. Fluorescence microscopy yog siv los kawm txog kev nqus thiab kev thauj mus los ntawm cov khoom hauv hlwb, kev faib tawm thiab thaj chaw ntawm cov tshuaj lom neeg, thiab lwm yam. Qee cov tshuaj hauv hlwb, xws li chlorophyll, tuaj yeem fluoresce tom qab raug irradiated los ntawm ultraviolet rays; Txawm hais tias qee yam khoom lawv tus kheej tsis tuaj yeem fluoresce, lawv tuaj yeem fluoresce tom qab stained nrog fluorescent dyes lossis fluorescent antibodies tom qab raug irradiated los ntawm ultraviolet rays. Fluorescence microscopy yog ib qho ntawm cov cuab yeej rau kev tshawb fawb zoo thiab ntau yam ntawm cov khoom no.
Optical microscope feem ntau muaj ib theem, lub teeb pom kev zoo, lub hom phiaj lens, lub qhov muag, thiab lub ntsej muag tsom. Los ntawm kev hloov txoj kev teeb pom kev zoo, lub teeb pom kev teeb pom kev zoo tuaj yeem tau txais cov kev soj ntsuam sib txawv xws li cov ntsiab lus tsaus nti ntawm qhov ci ci tom qab (hu ua lub teeb pom kev kaj lug) los yog cov ntsiab lus ci ntawm qhov tsaus ntuj (hu ua tsaus nti illumination), thiaj li nrhiav tau zoo dua. thiab saib xyuas microstructures nyob rau hauv ntau yam xwm txheej.
Fluorescence microscopes txawv ntawm cov tshuab microscopes zoo li hauv qab no:
1. Txoj kev illumination feem ntau yog episcopic, uas yog, lub teeb qhov chaw yog projected ntawm tus qauv los ntawm lub hom phiaj lens;
2. Lub teeb ci yog ultraviolet lub teeb, lub wavelength yog luv dua, thiab qhov kev daws teeb meem yog siab dua li ntawm cov microscopes zoo tib yam;
3. Muaj ob lub lim dej tshwj xeeb, ib qho nyob rau hauv pem hauv ntej ntawm lub teeb ci yog siv los lim tawm lub teeb pom kev, thiab ib qho nruab nrab ntawm lub qhov muag thiab lub hom phiaj lens yog siv los lim tawm ultraviolet rays los tiv thaiv tib neeg lub qhov muag.
Fluorescence microscope kuj yog ib yam ntawm optical microscope, qhov sib txawv tseem ceeb yog qhov excitation wavelength ntawm ob qhov sib txawv. Qhov no txiav txim siab qhov sib txawv ntawm fluorescence microscope thiab lub tshuab kho qhov muag zoo tib yam ntawm cov qauv thiab kev siv.
Fluorescence microscopy yog ib qho cuab yeej tseem ceeb hauv immunofluorescent cytochemistry. Nws yog tsim los ntawm cov khoom tseem ceeb xws li lub teeb ci, lim phaj system thiab optical system. Nws yog siv ib lub teeb ntawm lub teeb kom zoo siab rau cov qauv kom tawm fluorescence, thiab saib xyuas cov duab fluorescence ntawm cov qauv los ntawm kev nthuav dav lub hom phiaj lens thiab eyepiece system.
