Qhov txawv ntawm Fluorescence Microscopy thiab Confocal Laser Microscopy
Ua ntej, txoj ntsiab cai txawv
1. Fluorescence microscope: Nws siv lub teeb ci ultraviolet los ua lub teeb ci rau irradiate cov khoom los kuaj xyuas kom nws tawm fluorescence, thiab tom qab ntawd saib cov duab thiab qhov chaw ntawm cov khoom hauv qab lub tshuab tsom.
2. Laser confocal microscope: Lub laser scanning ntaus ntawv yog ntsia rau ntawm lub hauv paus ntawm fluorescence microscope imaging, thiab cov fluorescent sojntsuam zoo siab los ntawm ultraviolet lub teeb los yog pom lub teeb.
Qhov thib ob, cov yam ntxwv txawv
1. Fluorescence microscope: siv los kawm txog kev nqus, kev thauj mus los, kev faib tawm thiab thaj chaw ntawm cov tshuaj lom neeg hauv cov hlwb. Qee yam khoom hauv hlwb, xws li chlorophyll, tuaj yeem fluoresce tom qab irradiated los ntawm ultraviolet rays; Lwm yam khoom tsis tuaj yeem fluoresce los ntawm lawv tus kheej, tab sis yog tias lawv dyed nrog fluorescent dyes lossis fluorescent antibodies, lawv tuaj yeem fluoresce thaum irradiated los ntawm ultraviolet rays.
2. Laser confocal microscope: siv lub computer ua cov duab kom tau txais cov duab fluorescent ntawm sab hauv microstructure ntawm cov hlwb lossis cov ntaub so ntswg, thiab saib xyuas cov cim ntawm lub cev xws li Ca2 ntxiv, pH, daim nyias nyias muaj peev xwm thiab kev hloov pauv hauv cell morphology ntawm qib subcellular.
3. Kev siv sib txawv
1. Fluorescence microscopy: Fluorescence microscopy yog ib qho cuab yeej yooj yim rau immunofluorescence cytochemistry. Nws yog tsim los ntawm cov khoom tseem ceeb xws li lub teeb ci, lim phaj system thiab optical system. Nws siv ib lub teeb ntawm lub teeb los ua kom lub cev muaj zog kom tawm fluorescence, thiab nthuav nws los ntawm lub hom phiaj lens thiab lub qhov muag kom pom cov duab fluorescence ntawm cov qauv.
2. Laser confocal microscopy: Laser scanning confocal microscopy tau siv nyob rau hauv txoj kev tshawb no ntawm cell morphological localization, peb-dimensional qauv reorganization, dynamic hloov txheej txheem, thiab lwm yam, thiab muab tswv yim kev tshawb fawb txoj kev xws li quantitative fluorescence ntsuas thiab quantitative duab tsom xam, ua ke. nrog rau lwm yam technologies uas muaj feem xyuam rau biological technologies Nws tau dav siv nyob rau hauv lub teb ntawm molecular cell biology xws li morphology, physiology, immunology, noob caj noob ces, thiab lwm yam.
