Kev npaj thiab soj ntsuam lom neeg siv hluav taws xob microscopes
Kev daws teeb meem ntawm lub tshuab tsom iav nyob ntawm lub wavelength ntawm lub teeb siv. Lub tshuab hluav taws xob hluav taws xob, uas tau tshwm sim hauv xyoo 1933, siv cov kab hluav taws xob hluav taws xob nrog lub wavelength luv dua li pom lub teeb raws li nws lub teeb ci. Qhov no zoo heev txhim kho nws cov kev daws teeb meem piv nrog optical microscopes. Qhov sib txawv ntawm cov teeb pom kev zoo kuj ua rau muaj kev sib txawv ntawm electron microscopes thiab optical microscopes.
Raws li qhov sib txawv ntawm cov qauv siv hluav taws xob hauv hluav taws xob thiab txoj hauv kev uas cov nqaj cuam tshuam nrog cov qauv, niaj hnub electron microscopes tau hloov mus rau ntau hom. Cov khoom siv feem ntau yog kis tau tus mob electron microscopes (TEM) thiab scanning electron microscopes (SEM). Tag nrho qhov loj ntawm TEM tuaj yeem nyob ntawm 1000 txog 1,000,000 zaug, thaum lub sijhawm SEM yog li ntawm 20 txog 300,000 zaug. Qhov kev sim no feem ntau qhia txog cov qauv kev npaj rau kev xa xov hluav taws xob microscopes thiab scanning electron microscopes.
II. Apparatus thiab Materials
Lim:Escherichia colikab lis kev cai
Kev daws thiab cov tshuaj reagents: Formvar, concentrated sulfuric acid, absolute ethanol, sterile dej, 2% aqueous sodium phosphotungstate tov (pH 6.5-8.0), 0.3% polyvinyl formal solution (solved in chloroform), cytochrome C, ammonium acetate, 2BR.
Cov cuab yeej thiab cov khoom siv: Cov khoom siv kho qhov muag pom kev zoo, daim phiaj tooj liab, Buchner funnel, beaker, petri phaj, sterile dropper, sterile forceps, dissecting koob, iav swb, hemocytometer, nqus evaporator, lub tshuab ziab khaub ncaws tseem ceeb, thiab lwm yam.
III. Cov txheej txheem ua haujlwm
(1) Kev Npaj Ua Qauv thiab Kev Pom Zoo rau Kev Tshaj Tawm Electron Microscopy
Kev kho cov kab hlau hlau Cov qauv rau kho qhov muag microscopy raug soj ntsuam ntawm cov iav slides. Txawm li cas los xij, rau kev xa hluav taws xob microscopy, cov hluav taws xob tsis tuaj yeem nkag mus rau iav, yog li mesh substrates, feem ntau hu ua cov kab sib txuas, yog siv los hloov. Specimen grids muaj nyob rau hauv ntau yam specifications raws li cov khoom thiab cov duab. Daim phiaj tooj liab ntawm 200-400 mesh yog siv dav tshaj plaws. Daim phiaj tooj liab yuav tsum tau muab ntxuav ua ntej siv los tshem tawm cov kab mob, txwv tsis pub qhov zoo ntawm cov yeeb yaj kiab txhawb nqa thiab qhov pom tseeb ntawm cov qauv micrographs yuav raug cuam tshuam. Qhov kev sim no siv 400-mesh tooj liab daim phiaj, kho raws li hauv qab no: thawj zaug soak hauv Formvar tov rau ob peb teev, yaug dua nrog dej distilled, ces dehydrate los ntawm soaking nyob rau hauv kiag li ethanol. Yog tias daim phiaj tseem qias neeg tom qab cov kauj ruam saum toj no, muab tso rau hauv diluted concentrated sulfuric acid (1: 1) rau 1-2 feeb, los yog boil rau hauv 1% NaOH tov rau ob peb feeb. Yaug kom huv si nrog dej distilled, dehydrate nyob rau hauv kiag li ethanol, thiab muab tso tseg rau tom qab siv.
