Qhov zoo ntawm Multiphoton Laser Scanning Microscopy: Ib qho luv luv
1. Zoo siab los ntawm lub teeb liab lossis infrared, lub teeb tawg yog me me, thiab qhov tawg ntawm cov khoom me me yog qhov sib npaug ntawm plaub lub zog ntawm lub wavelength.
2. Ntau cov photons tawg los ntawm cov duab hla ntu tuaj yeem sau tau yam tsis xav tau cov pinholes.
3. Pinholes tsis tuaj yeem paub qhov txawv txav ntawm cov photons tawm ntawm qhov chaw defocused los yog focal, thiab multiphotons muaj cov teeb liab-rau-nruab sib piv zoo dua hauv cov duab sib sib zog nqus.
4. Lub teeb ci ultraviolet los yog pom pom siv rau ib qho photon excitation yog yooj yim absorbed thiab attenuated los ntawm cov qauv ua ntej lub beam mus txog lub focal dav hlau, ua rau nws nyuaj rau excite sib sib zog nqus txheej.
5. Nyob rau hauv cov nqe lus ntawm kev tshuaj ntsuam xyuas kab mob, thawj qhov kev txiav txim siab yog kom tsis txhob ua kom lub cev ua haujlwm ntawm lub cev nws tus kheej, tswj cov dej, ion concentration, oxygen, thiab cov as-ham. Hauv kev soj ntsuam lub teeb, ob qho tib si thermal thiab photon zog yuav tsum nyob twj ywm nyob rau hauv lub irradiation koob tshuaj thiab lub teeb lub zog uas tsis ua puas hlwb.
6. Multiphoton microscopy muaj ntau yam zoo. Nyob rau hauv cov nqe lus ntawm peb-dimensional daws teeb meem, qhov tob invasion, scattering efficiency, tom qab lub teeb, teeb liab-rau-suab nrov piv, tswj, thiab lwm yam., muaj cov yam ntxwv uas yav dhau los laser microscopes tsis muaj los yog tsis tau tshaj
Disadvantages ntawm multiphoton laser scanning microscope:
1. Tsuas yog rau cov duab fluorescence xwb.
2. Yog tias tus qauv suav nrog chromophores uas tuaj yeem nqus tau lub teeb pom kev zoo, cov qauv yuav raug thermal puas.
3. Qhov kev daws teeb meem yog txo qis me ntsis, txawm hais tias nws tuaj yeem txhim kho los ntawm ib txhij siv confocal qhov me me, muaj teeb liab poob.
4. Vim muaj cov kev txwv ntawm kim ultrafast lasers, tus nqi ntawm multiphoton scanning microscopes kuj yog siab.
