Qhov zoo ntawm Confocal Laser Scanning Microscopy hauv Kev Tshawb Fawb Txog Kev Noj Qab Haus Huv
Piv nrog cov tshuaj kho qhov muag tsom iav, confocal laser scanning microscopes muaj kev daws teeb meem siab dua, tso cai rau kev soj ntsuam ib txhij ntawm ntau lub teeb liab fluorescence, thiab tuaj yeem tsim cov duab peb sab. Hauv kev soj ntsuam ntawm cov qauv lom neeg, confocal laser scanning microscopy muaj cov hauv qab no zoo:
Kev soj ntsuam tas li ntawm cov ntaub so ntswg nyob, cov hlwb lossis cov seem ntawm tes ua rau kom tau txais cov duab peb sab ntawm cov cytoskeleton, chromosomes, organelles thiab cell membrane systems.
Nws muab cov duab nrog qhov sib txawv thiab kev daws teeb meem ntau dua li cov pa fluorescence microscopes, thaum muab siab rhiab heev thiab kev tiv thaiv zoo rau cov qauv.
Tau txais cov duab multi-dimensional. Nws txhawb 7-dimensional imaging (XYZaλIt), suav nrog XYT, XZT thiab XT scanning, time-series scanning, rotational scanning, regional scanning thiab spectral scanning; kev ua duab kuj tuaj yeem ua tau yooj yim.
Intracellular ion fluorescence labeling. Ib qho lossis ntau daim ntawv sau npe tuaj yeem siv los txheeb xyuas thiab saib xyuas cov pH ntawm lub cev nrog rau qhov sib piv ntawm cov concentration thiab kev hloov pauv ntawm ions xws li sodium, calcium thiab magnesium.
Fluorescence labeling kev soj ntsuam. Nws tso cai rau kev soj ntsuam cov tshuaj fluorescently sau cov tshuaj lom neeg nyob hauv cov hlwb lossis cov ntaub so ntswg, suav nrog cov cim ntawm daim nyias nyias, tshuaj lom neeg cov tshuaj tiv thaiv, receptors thiab ligands, nucleic acids thiab lwm yam khoom. Ntau yam khoom ntawm tib tus qauv tuaj yeem sau npe thiab pom ib txhij.
Kev kuaj pom ntawm tes yog tsis muaj kev cuam tshuam, ruaj khov, txhim khu kev qha thiab rov ua dua. Txhua cov ntaub ntawv thiab cov duab tuaj yeem raug xa tawm hauv lub sijhawm tiag tiag lossis khaws cia ntev.
