Qhov zoo thiab qhov txwv ntawm Multiphoton Laser Scanning Microscopy
1. Zoo siab los ntawm lub teeb liab los yog infrared, lub teeb tawg yog me me (qhov tawg ntawm cov khoom me me yog inversely proportional rau plaub lub zog ntawm lub wavelength).
2. Tsis xav tau pinholes, muaj peev xwm sau tau ntau qhov tawg ntawm photons los ntawm cov duab hla ntu.
3. Pinholes tsis tuaj yeem paub qhov txawv txav ntawm cov photons tawm ntawm qhov chaw defocused los yog focal, thiab multiphotons muaj cov teeb liab-rau-nruab sib piv zoo dua hauv cov duab sib sib zog nqus.
4. Lub teeb ci ultraviolet los yog pom pom siv rau ib qho photon excitation yog yooj yim absorbed thiab attenuated los ntawm cov qauv ua ntej lub beam mus txog lub focal dav hlau, ua rau nws nyuaj rau excite sib sib zog nqus txheej.
5. Nyob rau hauv cov nqe lus ntawm biological microscopy soj ntsuam, thawj qhov kev txiav txim siab yog kom tsis txhob ua puas lub active lub xeev ntawm lub cev nws tus kheej, thiab tswj cov kev ncig ntawm dej, ion concentration, oxygen, thiab cov as-ham. Hauv kev soj ntsuam lub teeb, ob qho tib si thermal thiab photon zog yuav tsum nyob twj ywm nyob rau hauv lub irradiation koob tshuaj thiab lub teeb lub zog uas tsis ua puas hlwb.
6. Multiphoton microscopy kuj muaj ntau yam zoo. Nyob rau hauv cov nqe lus ntawm peb-dimensional daws teeb meem, qhov tob ntxeem tau, tawg efficiency, tom qab lub teeb, teeb liab-rau-suab nrov piv, tswj, thiab lwm yam., laser microscopes muaj unparalleled los yog superior yam ntxwv uas yav dhau los laser microscopes tsis muaj.
Multiphoton confocal laser scanning microscope tau txuas ntxiv mus rau ntau yam kev tshawb fawb thiab daim ntawv thov teb Nws tuaj yeem ua peb-dimensional tsis-kev soj ntsuam ntawm cov qauv hauv lawv lub xeev ntuj thiab txhim kho cov kev daws teeb meem thiab teeb liab-rau-suab nrov piv ntawm cov kab ke Los ntawm kev siv cov kev hloov pauv hauv cov khoom siv tom qab multiphoton excitation, peb-dimensional siab cov ntaub ntawv khaws cia thiab peb-dimensional muaj peev xwm ua tiav cov ntaub ntawv muaj nuj nqis hauv microfas. ntseeg tau tias nrog kev txhim kho ntxiv ntawm cov tshuab, khoom siv, thiab laser technologies ntsig txog multiphoton confocal microscopy, multiphoton confocal laser scanning microscopy yuav muaj kev txhim kho ntau dua thiab siv dav dua.
Qhov tsis zoo yog raws li nram no:
1. Tsuas yog rau cov duab fluorescence xwb.
2. Yog tias cov qauv suav nrog chromophores uas tuaj yeem nqus tau lub teeb pom kev zoo, xws li cov xim pigments, cov qauv yuav raug thermal puas.
3. Qhov kev daws teeb meem tau txo qis me ntsis, txawm hais tias nws tuaj yeem txhim kho los ntawm ib txhij siv confocal qhov me me, yuav muaj teeb liab poob.
4. Vim muaj cov kev txwv ntawm kim ultrafast lasers, tus nqi ntawm multiphoton scanning microscopes kuj yog siab.
