Dab tsi yog qhov txawv ntawm fluorescence microscope thiab laser confocal microscope?
Muaj qhov sib txawv ntawm cov qauv kev ua haujlwm thiab kev siv ntawm ob. Nws tau piav raws li hauv qab no:
fluorescence microscope
1. Fluorescence microscope yog ib qho cuab yeej siv lub teeb ci ultraviolet los ua lub teeb ci los teeb pom kev ntawm cov khoom uas tau sim, ua rau nws tawm fluorescence, thiab tom qab ntawd saib cov duab thiab qhov chaw ntawm cov khoom hauv qab lub tshuab tsom. Fluorescence microscopy yog siv los kawm txog kev nqus, kev thauj mus los, kev faib tawm, thiab chaw nyob ntawm cov khoom hauv hlwb. Qee yam khoom hauv hlwb, xws li chlorophyll, tuaj yeem tawm fluorescence tom qab raug ultraviolet hluav taws xob; Qee cov tshuaj lawv tus kheej yuav tsis tawm fluorescence, tab sis yog stained nrog fluorescent dyes lossis fluorescent antibodies, lawv tuaj yeem tawm fluorescence nyob rau hauv ultraviolet hluav taws xob. Fluorescence microscopy yog ib qho ntawm cov cuab yeej rau kev tshawb fawb zoo thiab muaj nuj nqis ntawm cov tshuaj no.
2. Lub hauv paus ntsiab lus ntawm fluorescence microscope:
(A) Lub teeb qhov chaw: Lub teeb ci emits lub teeb ntawm ntau lub wavelengths (los ntawm ultraviolet mus rau infrared).
(B) Excitation filter light source: dhau los ntawm ib lub wavelength tshwj xeeb ntawm lub teeb uas tuaj yeem tsim cov fluorescence hauv cov qauv, thaum thaiv
Thaiv lub teeb tsis muaj txiaj ntsig uas txhawb nqa fluorescence.
(C) Fluorescent specimens: feem ntau stained nrog fluorescent pigments.
(D) Blocking filter: xaiv kis fluorescence los ntawm thaiv lub teeb excitation uas tsis tau nqus los ntawm cov qauv, thiab qee qhov wavelengths kuj tau xaiv hauv fluorescence. Lub microscope uas siv lub teeb ultraviolet ua lub teeb ci los tawm fluorescence los ntawm cov khoom irradiated. Lub tshuab ntsuas hluav taws xob tau tsim thawj zaug los ntawm Knorr thiab Harroska hauv Berlin, lub teb chaws Yelemees xyoo 1931. Hom tshuab ntsuas no siv lub tshuab hluav taws xob siab ceev hloov lub teeb ntawm lub teeb. Vim qhov luv luv wavelength ntawm cov electron txaus piv rau lub teeb nthwv dej, qhov magnification ntawm electron microscope tuaj yeem ncav cuag 800000 zaug, nrog qhov kev daws teeb meem tsawg kawg nkaus ntawm 0.2 nanometers. Lub tshuab ntsuas hluav taws xob tshuab hluav taws xob, uas tau pib siv xyoo 1963, tso cai rau tib neeg pom cov qauv me me ntawm cov khoom.
3. Daim Ntawv Thov: Siv los nthuav cov duab ntawm cov khoom me me. Feem ntau siv rau kev soj ntsuam ntawm biology, tshuaj, microscopic hais, thiab lwm yam.
