Dab tsi yog qhov txawv ntawm fluorescence microscopy thiab inverted microscopy
Microscope yog ib qho cuab yeej tseem ceeb hauv kab lis kev cai ntawm tes thiab lwm yam kev sim derivative. Tam sim no, muaj ntau hom kab mob hauv lub khw, thiab xaiv lub tshuab tsom iav uas ua tau raws li qhov xav tau thiab tsim nyog yog qhov nyuaj. Hauv qab no, peb yuav qhia cov ntsiab lus ntawm inverted microscopes thiab fluorescence microscopes rau txhua tus xaiv los ntawm.
Lub inverted microscope, zoo li ib lub tshuab microscope, feem ntau muaj peb ntu: txhua yam, qhov teeb pom kev zoo, thiab qhov muag pom.
Cov muaj pes tsawg leeg ntawm lub tshuab ntsuas inverted yog tib yam li ntawm lub tshuab ntsuas lub ntsej muag tsis tu ncua, tshwj tsis yog tias lub hom phiaj lens thiab teeb pom kev zoo yog thim rov qab, nrog rau yav dhau los nyob rau hauv theem thiab tom qab saum toj ntawm theem.
Cov qauv no nthuav dav qhov kev ncua deb ntawm lub teeb pom kev zoo ntawm lub teeb pom kev zoo thiab theem, ua rau nws yooj yim dua los tso cov cuab yeej saib xyuas kom yooj yim xws li kab lis kev cai tais diav thiab cell kab lis kev cai fwj (ntawm chav kawm, iav slides kuj siv tau), thaum lub sij hawm ua hauj lwm nyob deb ntawm lub Lub hom phiaj lens thiab cov khoom siv tsis tas yuav tsum loj heev.
Inverted microscope yog siv los ntawm cov tsev kho mob thiab kev noj qab haus huv, cov tsev kawm qib siab, thiab cov chaw tshawb fawb soj ntsuam cov kab mob, cov kab mob, cov kab mob, cov kab mob hauv cov ntaub so ntswg, ncua kev kawm, cov sediments, thiab lwm yam. tuaj yeem ntes txhua daim ntawv ntawm cov txheej txheem no.
Siv dav hauv kev lag luam xws li cytology, parasitology, oncology, immunology, genetic engineering, industrial microbiology, thiab botany.
Fluorescence microscopy yog siv los kawm txog kev nqus, kev thauj mus los, kev faib tawm, thiab chaw nyob ntawm cov khoom hauv hlwb.
Rau cov khoom kuaj, muaj ob txoj hauv kev los tsim cov fluorescence: spontaneous fluorescence, uas yog ncaj qha tawm los ntawm ultraviolet irradiation; Secondary fluorescence tshwm sim thaum cov khoom pom tau kho nrog fluorescent dyes thiab raug ultraviolet lub teeb ua ntej emitting fluorescence.
Qee yam khoom hauv hlwb, xws li chlorophyll, tsim cov fluorescence tshwm sim tom qab raug ultraviolet hluav taws xob; Qee cov tshuaj lawv tus kheej yuav tsis tawm fluorescence, tab sis yog stained nrog fluorescent dyes los yog fluorescent antibodies, lawv kuj tuaj yeem tawm cov fluorescence thib ob hauv qab hluav taws xob ultraviolet.
Lub fluorescence microscope siv lub teeb pom kev zoo ntawm lub teeb pom kev zoo kom tso tawm qee qhov wavelength ntawm lub teeb (UV 365nm lossis UV xiav 420nm) los ntawm cov xim lim dej ua lub teeb pom kev zoo, uas ua rau cov tshuaj fluorescent hauv cov qauv kom tawm ntau xim ntawm fluorescence. Tom qab ntawd, nws raug pom los ntawm kev nthuav dav ntawm lub hom phiaj lens thiab eyepiece.
Nyob rau hauv txoj kev no, txawm tias tsis muaj zog fluorescence, nws yog ib qho yooj yim pom thiab rhiab heev nyob rau hauv muaj zog contrasting keeb kwm. Nws yog tsuas yog siv rau kev kawm cell qauv, muaj nuj nqi, thiab tshuaj muaj pes tsawg leeg.
