Qhov kev npaj txoj kev ntawm lub tshuab tsom me me
1. Hniav txoj kev
Smearing txoj kev yog ib txoj kev npaj swb los ntawm cov khoom siv cov txheej txheem rau lawv.
Cov ntaub ntawv smear muaj xws li - Algae, cov nqaij ntshiv me me, cov ntshav, kuaj, anthers, thiab lwm yam.
Thaum smearing, saib yuav tsum tau them rau:
(1) Daim iav xaub yuav tsum raug ntxuav.
(2) Cov kab xaub iav yuav tsum ua tiaj.
(3) Cov txheej yuav tsum muaj cov khaub ncaws hnav. Thov siv lub droplet rau nruab nrab txoj cai ntawm lub khob xaub thiab kis nws tusyees nrog tus hniav lossis hniav sib tsoo.
(4) Cov txheej yuav tsum yog nyias. Siv lwm lub xaub iav uas yog swb, maj mam thawb los ntawm ob sab ntawm lub khob xaub (lub kaum sab xis yuav tsum muaj 30℃rau 45℃txheej.
(5) tsau. Yog tias kev txhim kho yog qhov yuav tsum tau, cov txheej txheem tshuaj lossis cov txheej txheem kom qhuav (cov kab mob) tuaj yeem siv rau kev kho kom yooj yim.
(6) staining. Cov kab mob siv methylene xiav, thiab cov ntshav siv wright kev daws teeb meem. Qhov kev daws qhov quav txias yuav tsum tau npog tag nrho cov txheej sib txawv.
(7) yaug. Siv daim ntawv nqus kom nqus tau lossis qhuav.
(8) Kaw cov yeeb yaj kiab. Lub sijhawm ntev kev khaws cia siv cov yeeb yaj kiab ntoo canadian ntoo.
2. Ntsiav tshuaj nias txoj kev
Txoj kev compression yog ib txoj kev ntawm cov ntaub ntawv biological los ntawm kev tso lawv ntawm ib lub khob xaub thiab npog, thov ib lub siab tsis txhob tso cov ntaub so ntswg.
Cov txheej txheem dav dav ntawm cov ntsiav tshuaj nias txoj kev:
(1) Xaiv cov khoom siv. Txhawm rau saib xyuas cov kab mob hauv tes, cov nqaij tshiab nrog cov khoom muaj zog ntawm kev sib faib ntawm tes yuav tsum tau xaiv ua cov khoom siv. Xws li cov taub hau hauv paus, qia taub meristem, pob txha pob txha pob txha, anthers (pollen niam hlwb). Phev zes (spermatocytes), thiab lwm yam.
(2) tsau. Cov khoom nruab yooj yim tuaj yeem txiav txim siab raws li qhov xav tau, thiab cov qauv yuav tsum tau muab tso rau tam sim ntawd thiab pom. Nws tsis tsim nyog kom ua cais kho kev kho mob (synchronous nrog staining); Yog tias cov khoom siv tsis tau kuaj xyuas tam sim ntawd tom qab kuaj, nws tuaj yeem kho nrog kev txhim kho nrog kev txhim kho (feem ntau acetic acid Consative). Tom qab kho rau 2-24 teev (nyob ntawm cov khoom), yaug nrog 95% ethanol thiab khw hauv 70% ethanol rau tom qab siv.
(3) sib cais. Cov ntaub ntawv uas tsis yooj yim tawg los ntawm cov hlwb yuav tsum tau kho nrog kev daws cov kua roj hydrolysis (xws li 1n hcrod acid semen) rau 6-20 feeb. Tom qab kev sib cais, lawv yuav tsum yaug nrog dej ua ntej staining.
(4) staining. Muaj ntau hom dyes. Chromosomes feem ntau tsuas yog stained nrog acetic anid magenta staining daws.
(5) nias cov ntsiav tshuaj. Tso cov khoom siv rau ntawm lub khob swb, ntxiv cov dej ntws lossis zas xim, npog cov xaub nrog npog, thiab maj mam nias nws nrog koj tus ntiv tes xoo.
(6) saib xyuas. Tom qab compression, nws tuaj yeem pom nyob rau hauv lub tshuab tsom.
