Cov txheej txheem ntawm kev siv cov pH meter hauv kev sim cov khoom siv mis nyuj
Yuav ua li cas ntxuav cov khoom noj siv mis tom qab ntsuas acidity, thiab yuav ua li cas ntxuav lawv rau cov protein ntau thiab cov protein ntau. Cov qib ntawm electrode paug sib txawv thaum ntsuas cov xov xwm sib txawv lossis ua haujlwm nyob rau hauv ib puag ncig sib txawv, yog li txoj kev ntxuav yuav raug tshuaj xyuas los ntawm rooj plaub. Muaj ntau ntau hom kev tu rau pH ntsuas. Kev ceev faj yuav tsum tau them thaum lub sijhawm txheej txheem, vim tias iav pH electrodes feem ntau yog siv los ntsuas cov tshuaj no. Ntxuav cov sediment sab laug ntawm lub electrode:
1, Thaum ntsuas cov protein hauv cov khoom noj siv mis:
So hauv hydrochloric acid tov ntawm gastric protease (HCl concentration ntawm 0.1mol/L) rau 15 feeb, los yog tsau nyob rau hauv 10% sodium hypochlorite tov rau 5 feeb. Tom qab ntawd, ntxuav nrog dej ntshiab thiab tsau rau hauv 3.3mol / L KCl electrode soaking tov rau 12 teev kom muaj peev xwm sib npaug ntawm electrode. Qhov no tuaj yeem rov qab ua nws cov haujlwm qub. Yog tias tsis yog, nws nyob ntawm seb lub electrode puas yuav tsum tau hloov;
2, Thaum ntsuas cov protein ntau thiab cov khoom noj muaj roj ntau:
Soak cov electrode hauv cov tshuaj ntxuav tais diav thiab tuav rau 1 teev. Tshem tawm cov electrode thiab yaug kom huv si, tom qab ntawd tsau nws hauv cov tshuaj gastric protease rau 4 teev. Txoj kev npaj ntawm gastric protease tov: Sib tov 5% gastric protease nyob rau hauv 0.5mol / L hydrochloric acid tov nyob rau hauv ib tug ratio ntawm 1: 1 * Tom qab ntawd, tshem tawm cov electrode, ntxuav nws nrog distilled dej, thiab tsau nyob rau hauv ib tug 3mol. /L (ntau dua, 3.3mol) KCL tov rau tom qab siv. Cov electrode kuj yuav tsum tau ntim nrog 3mol / L KCL tov kom tsis txhob qhuav
Cov khoom noj mis nyuj muaj cov yam ntxwv ntawm cov protein ntau thiab cov roj ntau, uas tuaj yeem ua rau muaj kev cuam tshuam ntawm electrode iav pob capillaries. Ib qho electrode nyob rau hauv ib txwm siv yuav muaj kev teb qeeb thiab kev nyeem tsis ruaj khov tom qab peb lub hlis; Txhawm rau txuag cov nqi siv thiab faib cov peev txheej tsim nyog.
Vim tias acidity meter tau dav siv hauv kev lag luam mis nyuj. Tshwj xeeb tshaj yog nyob rau hauv kev ntsuam xyuas ntawm ib nrab thiab tiav cov khoom, nws plays lub luag hauj lwm tseem ceeb heev, thiab tus nqi pH ntawm hom no qhia txog qhov zoo ntawm cov tshuaj ntsuam xyuas. Yog tias tus nqi pH tsis tuaj yeem ntsuas kom raug raws sijhawm rau kev siv khoom siv, nws yuav muaj kev cuam tshuam tsis zoo rau kev tsim khoom.
