Cov khoom tseem ceeb thiab kev ua haujlwm ntawm laser confocal microscope
1. Illuminating pinhole
Muaj nuj nqi: Los tsim lub teeb pom kev zoo tom qab lub laser dhau los ntawm lub teeb pom kev zoo, qhov chaw teeb pom kev zoo muaj qhov tshwj xeeb zoo ntawm cov kev taw qhia muaj zog ntawm lub teeb ci, me me dispersion, siab brightness, siab spatial thiab temporal coherence, thiab dav hlau polarization excitation. Thiab nrog tus ntes pinhole thiab lub dav hlau focal los tsim cov khoom siv confocal.
2.Beam Separator
Muaj nuj nqi: Cais cov qauv excitation fluorescence los ntawm lwm yam tsis-cim teeb.
3. Lub hom phiaj lens
4.Focal dav hlau
Kev Ua Haujlwm: Lub laser taw tes lub teeb qhov chaw irradiates cov khoom thiab tsom nws ntawm lub dav hlau focal, uas ua rau cov fluorescence ntawm cov fluorescently sau cov qauv kom tawm fluorescence thiab tsim ib qho chaw focal. Qhov chaw yog tom qab ntawd ua tiav los ntawm cov khoom siv xws li lub hom phiaj lens, beam splitter, thiab lwm yam, thiab tsom mus rau lub teeb pom kev zoo pinhole thiab lub ntsuas ntsuas pinhole raws li. Qhov no yog qhov uas lub sij hawm confocal los ntawm.
5. Ntes pinhole
Function: Ua raws li cov lim dej spatial. * Zoo heev impede lub dav hlau tsis-focusing tawg ntho lub teeb thiab lub dav hlau tsom ntawm qhov chaw uas tsis yog-focal chaw sab nraum lub tawg tawg lub teeb kom ntseeg tau tias cov fluorescence teeb liab tau txais los ntawm lub detector pinhole tag nrho los ntawm cov qauv chaw tsom xam txoj hauj lwm, yog li cov qauv ntawm lub diffraction. aggregation spot thiab detector pinhole imaging spot muaj tib cov ntaub ntawv (ob-point conjugate).
6. Photomultiplier tube (kwv yees)
Muaj nuj nqi: Txais lub teeb liab los ntawm lub pinhole, hloov nws mus rau hauv lub teeb liab hluav taws xob thiab xa mus rau lub computer, qhov twg cov duab meej ntawm tag nrho lub dav hlau focal tshwm ntawm qhov screen.
7. Laser: Txoj kev loj hlob ntawm confocal microscope technology tsis tuaj yeem sib cais los ntawm kev loj hlob sai ntawm lasers. Peb tuaj yeem xaiv cov lasers sib txawv raws li kev tshawb fawb xav tau. Xws li ArUV (351.364nm), HeCd (442nm), AR (457,488,514nm), ArKr (488,568,647nm) Kr (568nm), HeNe (543nm), HeNe (633nm) thiab lwm yam.
8.Multi-fluorescence channels: nrog ntau cov fluorescence raws kom ua tiav ntau daim ntawv lo ntawm cov qauv tib lub sijhawm.
