Taw qhia rau cov hauv paus ntsiab lus ntawm fluorescence microscopy
1, fluorescence microscope yog siv lub teeb ci ultraviolet ua lub teeb ci, siv rau irradiate cov khoom los kuaj xyuas, kom nws tawm fluorescence, thiab tom qab ntawd saib cov duab ntawm cov khoom thiab nws qhov chaw nyob hauv qab lub tshuab tsom. Fluorescence microscope yog siv los kawm txog kev nce thiab thauj cov khoom hauv lub cell, kev faib tawm thiab hauv cheeb tsam ntawm cov tshuaj, thiab lwm yam. Qee cov tshuaj hauv lub cell, xws li chlorophyll, tuaj yeem pom hauv cell. Qee yam khoom hauv lub cell, xws li chlorophyll, tuaj yeem fluoresce tom qab irradiation los ntawm lub teeb ultraviolet; muaj qee yam khoom uas tsis tuaj yeem fluoresce los ntawm lawv tus kheej, tab sis yog stained nrog fluorescent dyes los yog fluorescent antibodies, lawv kuj tuaj yeem fluoresce tom qab irradiation los ntawm ultraviolet lub teeb, thiab fluorescence microscope yog ib qho ntawm cov cuab yeej los ua cov kev tshawb fawb zoo thiab ntau yam ntawm cov khoom no.
2, Lub hauv paus ntsiab lus ntawm fluorescence microscope:
(A) lub teeb qhov chaw: lub teeb qhov chaw radiates ntau wavelengths ntawm lub teeb (nyob rau hauv ultraviolet rau infrared).
(B) excitation lim qhov chaw: los ntawm cov qauv tuaj yeem tsim cov fluorescence ntawm lub teeb tshwj xeeb ntawm lub teeb, thaum thaiv qhov excitation ntawm fluorescence tsis siv lub teeb.
(C) Cov qauv fluorescent: feem ntau stained nrog fluorochrome.
(D) Thaiv cov ntxaij lim dej: thaiv tawm lub teeb pom kev zoo uas tsis nqus los ntawm cov qauv kom xaiv cov fluorescence, thiab qee qhov wavelengths hauv cov fluorescence kuj tau xaiv kis.
Lub microscope uas siv lub teeb ultraviolet ua lub teeb ci los ua cov khoom irradiated fluoresce. Lub tshuab ntsuas hluav taws xob tau tsim thawj zaug hauv xyoo 1931 hauv Berlin, Lub Tebchaws Yelemees los ntawm Knorr thiab Haroska. Lub tshuab ntsuas hluav taws xob no siv lub teeb hluav taws xob hluav taws xob ceev ceev tsis yog lub teeb ci. Vim hais tias lub wavelength ntawm lub electron kwj yog luv dua lub teeb yoj, yog li qhov magnification ntawm electron microscope tuaj yeem ncav cuag 800,000 zaug, qhov tsawg kawg nkaus kev daws teeb meem ntawm 0.2 nm. Xyoo 1963 tau pib siv lub tshuab ntsuas hluav taws xob hauv tshuab hluav taws xob tuaj yeem ua rau tib neeg pom lub ntsej muag ntawm cov khoom me me.
3, Scope ntawm daim ntawv thov: siv los nthuav cov duab ntawm cov khoom me me. Feem ntau siv hauv biology, tshuaj, microscopic hais thiab lwm yam kev soj ntsuam.
