Kev siv tshuaj lom neeg - Laser Confocal Microscope
1. Muaj feem xyuam rau cell biology, cell physiology, neurobiology thiab neurophysiology thiab yuav luag txhua yam kev tshawb fawb ntawm tes.
2. Kev soj ntsuam ntawm lub sijhawm tiag tiag uas tsis muaj kev puas tsuaj thiab kev soj ntsuam ntawm cov hlwb nyob, thiab kev tshawb fawb txog kev ua haujlwm morphological thiab kev ua haujlwm. Non-invasive, txhim khu kev qha thiab reproducible cell nrhiav tau; cov ntaub ntawv dluab tuaj yeem tso tawm hauv lub sijhawm lossis khaws cia rau lub sijhawm ntev.
3. Nruam tomography ntawm cov cell nyob thiab cov ntaub so ntswg los yog cov ntaub so ntswg ntawm tes, tuaj yeem tau txais ib lub xovtooj ntawm tes zoo lossis ib pawg ntawm cov hlwb lossis cov qauv ntawm cov ntaub so ntswg hauv zos hauv txhua theem (nrog rau ob sab thiab peb sab) (nrog rau cov qauv tshwj xeeb ntawm tes - xws li cytoskeleton, chromosomes, organelles thiab cell membrane system, cov qauv ntawm cov qauv sib sib zog nqus) thiab ua tiav peb-dimensional duab (xws li kev tsom xam ntawm cov kev hloov ntawm lub sij hawm), piv txwv li, plaub-dimensional duab, tab sis kuj los soj ntsuam cov hloov raws sijhawm. (xws li, txheeb xyuas cov kev hloov pauv hauv lub sijhawm, piv txwv li, cov duab plaub-dimensional, tab sis kuj hloov pauv cov fluorescence wavelengths, uas tso cai rau ntau cov duab ntau yam). Nrhiav qhov chaw ntawm lub hlwb ntawm cov ntaub so ntswg thiab lwm cov qauv ntawm cov khoom yuav tsum tau ua raws li kev soj ntsuam ntawm lub sijhawm tiag tiag, kev tshuaj xyuas thiab kev sau cia; txheeb xyuas qhov zoo, qhov ntau, lub sijhawm thiab qhov chaw faib khoom.
4. Fluorescent labeling probe labeling of live cells or sliced specimens for soj ntsuam cellular biological substances, membrane labelling, cell tracing, substances, reactions, receptors or ligands, nucleic acids, etc.; ib txhij ntau yam tshuaj labeling thiab kev soj ntsuam ib txhij tuaj yeem ua tiav ntawm tib tus qauv.
5. Intracellular ion fluorescence labelling, ib leeg lossis ntau daim ntawv lo, nrhiav kom pom ntawm intracellular xws li pH thiab sodium, potassium, calcium, magnesium thiab lwm yam ions concentration piv ntsuas thiab nws cov kev hloov pauv hloov;
6. Kev ntsuas ntawm cell membrane muaj peev xwm, nrhiav kom tau dawb radicals, thiab lwm yam;
7. Ua qhov chaw fluorescence bleaching rov thwmsim, ua ke nrog fluorescence poob hauv fluorescence bleaching thwmsim, kawm intercellular kev sib txuas lus thiab lwm yam intracellular tshuaj (molecules, thiab lwm yam) zog; nyob rau hauv lub sij hawm-scanning thwmsim thiab yees duab-bleaching (lub teeb quenching) thwmsim, yuav ua tau ib simultaneous tso zis thiab hloov dua siab tshiab ntawm cov ntaub ntawv thiab cov duab rau txhua channel. Ua fluorescence resonance zog hloov kev sim los kawm txog kev txav ntawm cov molecules thiab ions nyob rau hauv lub cell los ntawm kev hloov ntawm fluorescence wavelength thiab kev sib cuam tshuam.
8. Nws yog qhov tseeb heev (spatial positioning, quantification, fixed wavelength, fixed time), rhiab, ceev thiab muaj peev xwm ua kom tiav qhov kev sib cais thiab kev soj ntsuam thiab kev soj ntsuam ntawm ntau lub wavelength dluab ntawm ntau daim ntawv fluorescent ntawm cov ntaub so ntswg ntawm tes tib lub sijhawm (txawm ntau yam fluorescents uas nws emission wavelengths nyob ze heev rau ib leeg, piv txwv li, qhov sib txawv ntawm tsuas yog ob peb nm), raws li zoo raws li on-line ntsuas thiab tsom xam muaj nuj nqi ntawm co-localisation ntawm ntau fluorescent daim ntawv lo.
