Vim li cas cov duab zoo ntawm laser confocal microscope zoo dua?
Lub hauv paus ntsiab lus ntawm laser confocal microscope yog tias lub teeb beam emissions los ntawm LED lub teeb qhov chaw hla los ntawm ib tug ntxeem tau disk thiab lub hom phiaj lens thiab ces tsom rau ntawm qhov chaw ntawm cov qauv. Tom qab ntawd, lub teeb beam yog reflected rov qab mus rau qhov ntsuas system los ntawm tus qauv nto. Thaum dhau los ntawm tus pinhole ntawm MPD dua, lub teeb pom kev zoo tsuas yog khaws qhov chaw tsom. Thaum kawg, lub beam yog reflected los ntawm lub beam splitter thiab duab ntawm lub koob yees duab.
1. Laser scanning tshuab yog siv nyob rau hauv laser confocal microscope. Piv nrog rau qhov dav-spectrum teeb pom kev zoo ntawm cov tshuaj microscope, laser scanning thev naus laus zis tuaj yeem txheeb xyuas qhov tseeb thiab tsom mus rau thaj chaw tshwj xeeb ntawm cov qauv, yog li txhim kho qhov kev daws teeb meem thiab qhov tseeb ntawm cov duab. Tib lub sijhawm, laser scanning thev naus laus zis tuaj yeem tshem tawm qhov tawg thiab cov teeb liab tom qab hauv cov qauv, yog li kev txhim kho qhov sib piv ntawm cov duab. Nyob rau tib lub sijhawm, lub monochromaticity ntawm laser ua kom pom tseeb dua.
2. Lub laser confocal microscope muaj qhov loj optical aperture (lub peev xwm ntawm lub tshuab tsom kom tau txais lub teeb emitted los ntawm cov qauv) thiab lub hom phiaj ntawm cov lej aperture siab (qhov loj ntawm lub lens), uas ua rau cov duab pom tseeb thiab meej dua.
3. Laser confocal microscope siv lub photomultiplier raj nrog siab rhiab heev, uas muaj peev xwm qhia tau siab rhiab heev rau tsis muaj zog fluorescence signals, thiab kuj yuav tshem tawm cov suab nrov tom qab los ntawm nqaim lub excitation ntau thiab siv optical slices. * * Nruab nrog lub siab-rhiab heev photodiode ntes, lub laser confocal microscope tuaj yeem ntes tau cov teeb liab kho qhov muag thiab hloov mus rau cov teeb liab hluav taws xob.
Sib txawv ntawm kev soj ntsuam kho qhov muag ib txwm muaj, photodiode detector tuaj yeem ntes tau ib qho photon, ua kom pom tseeb dua thiab raug. Lub ntsuas ntsuas siab siab no tseem tuaj yeem tau txais cov duab meej nyob rau hauv cov teeb meem siv hluav taws xob tsawg.
