Vim li cas cov duab zoo ntawm laser confocal microscopy zoo dua?
Lub hauv paus ntsiab lus ntawm laser confocal microscopy yog hais tias lub teeb beam emissions los ntawm ib tug LED teeb qhov chaw kis tau los ntawm ib tug ntau lub qhov disk thiab lub hom phiaj lens, thiab yog teem rau saum npoo ntawm cov qauv. Tom qab ntawd, lub beam yog reflected rov qab mus rau qhov ntsuas system los ntawm qhov chaw ntawm tus qauv. Thaum dhau los ntawm cov pinholes ntawm MPD dua, lub teeb pom kev zoo tsuas yog khaws cov ntsiab lus teeb pom kev zoo xwb. Thaum kawg, lub teeb ntawm lub teeb yog reflected los ntawm ib tug splitter thiab duab ntawm lub koob yees duab.
Vim li cas cov duab zoo ntawm laser confocal microscopy zoo dua?
1. Lub laser confocal microscope siv laser scanning tshuab. Piv nrog rau qhov dav-spectrum teeb pom kev zoo ntawm cov tshuaj microscopes, laser scanning thev naus laus zis tuaj yeem txheeb xyuas qhov tseeb thiab tsom mus rau cov chaw tshwj xeeb ntawm cov qauv, yog li txhim kho qhov kev daws teeb meem thiab qhov tseeb ntawm cov duab. Lub caij no, laser scanning thev naus laus zis tuaj yeem tshem tawm kev tawg thiab cov cim keeb kwm yav dhau hauv cov qauv, yog li txhim kho qhov sib piv ntawm cov duab. Lub caij no, lub monochromaticity ntawm lub laser ua kom pom tseeb dua.
2. Laser confocal microscopy muaj qhov loj dua optical aperture (lub peev xwm ntawm lub tshuab tsom kom tau txais lub teeb emitted los ntawm cov qauv) thiab lub hom phiaj ntawm cov zauv aperture siab (qhov loj ntawm lub lens), ua kom pom tseeb thiab meej dua.
3. Lub laser confocal microscope siv lub raj photomultiplier rhiab heev raws li cov khoom kuaj pom, uas tuaj yeem ua rau muaj kev nkag siab zoo rau cov teeb liab fluorescence tsis muaj zog. Nws kuj tseem tuaj yeem tshem tawm cov suab nrov tom qab los ntawm kev txo qhov excitation ntau thiab siv optical slicing ** Nruab nrog cov khoom siv photodiode rhiab heev, lub laser confocal microscope tuaj yeem ntes cov teeb liab sai thiab raug thiab hloov mus rau cov teeb liab hluav taws xob.
Tsis zoo li kev soj ntsuam kho qhov muag ib txwm muaj, photodiode detectors tuaj yeem ntes tus neeg photons, ua rau cov duab pom ntau dua thiab raug. Qhov ntsuas ntsuas qhov ntsuas siab siab no tseem tuaj yeem tau txais cov duab meej nyob rau hauv cov xwm txheej tsis tshua muaj teeb pom kev zoo.
Lub VT6000 laser confocal microscope yog raws li conjugate confocal hauv paus ntsiab lus ntawm lub pinhole point lub teeb qhov chaw, nrog nanometer theem longitudinal daws teeb meem. Coupled nrog ib tug high-ceev scanning module, kev soj ntsuam software muaj ntau cheeb tsam thiab tsis siv neeg ntsuas zog, uas yuav ua tau sai thiab automated ntsuas. Nws kuj tseem muab cov kab ntawm contour loj tsis xws li qhov siab, qhov dav, thiab lub kaum sab xis kom pom qhov zoo ntawm qhov chaw.
