Vim li cas peb thiaj li yuav tsum siv cov lensless holographic microscopes
Cov tshuaj microscopes tuaj yeem saib xyuas qhov kev siv zog ntawm kev faib tawm thaum saib cov qauv tshuaj lom neeg. Nyob rau hauv lawv lub xeev ntuj, cov hlwb feem ntau nyob rau hauv lub xeev tsis muaj xim thiab pob tshab, xav tau phau ntawv staining thiab imaging los ntawm cov neeg kho tshuab tsom, uas ua rau lub sijhawm ua haujlwm tsis zoo. Tsis tas li ntawd, cov qauv kho qhov muag sab hauv ntawm lub tshuab tsom iav yog qhov nyuaj, thiab qee qhov chaw tsim khoom siv yog kim, uas tsis zoo rau kev lag luam.
Cov teeb meem nrog cov kev daws teeb meem ib txwm muaj
1. Slow imaging ceev: Thaum siv cov tshuaj microscopy rau imaging, phau ntawv los yog tsis siv neeg tsom xam yog yuav tsum tau mus nrhiav cov duab dav hlau, uas yog tsis conducive rau real-time kev soj ntsuam ntawm biological kuaj.
2. Tus nqi kim: Cov kab ke niaj hnub muaj cov txheej txheem kho qhov muag nyuaj, thiab qee lub tshuab tsom iav yog kim, uas tsis tuaj yeem ua tau raws li kev xav tau ntawm kev lag luam hauv thaj chaw tsis muaj kev tsim kho.
3. Kev puas tsuaj ntawm tes: Ib txwm siv fluorescence microscopy yuav tsum staining ntawm cov hlwb ua ntej txhawm rau txhim kho cov duab zoo thaum soj ntsuam cov qauv tshuaj lom neeg, uas yuav txo qis kev ua haujlwm ntawm tes thiab ua rau cov cell puas.
Hauv cov txheej txheem tshawb nrhiav lub sijhawm tiag tiag ntawm cov qauv tshuaj lom neeg, lub lensless holographic microscope tuaj yeem siv los ua kom tiav cov duab peb sab ntawm lub sijhawm tiag tiag yam tsis tas yuav tsum tau ua ua ntej ntawm cov qauv tshuaj lom neeg (xws li staining). Cov duab reconstructed ntawm lub lensless holographic microscope tuaj yeem tsim kho dua tshiab siv cov duab kos duab algorithms, uas tuaj yeem ua tiav ib txhij ua tiav qhov loj ntawm lub kaum sab xis thiab siab daws teeb meem, ua tau raws li cov neeg siv khoom xav tau.
Cov yam ntxwv ntawm scanning electron microscopy
Piv nrog rau optical microscopy thiab kis electron microscopy, scanning electron microscopy muaj cov yam ntxwv hauv qab no:
(1) Cov qauv saum npoo ntawm cov qauv tuaj yeem pom ncaj qha, thiab qhov loj ntawm cov qauv tuaj yeem loj li 120mm x 80mm x 50mm.
(2) Cov txheej txheem kev npaj ua qauv yog yooj yim thiab tsis tas yuav txiav rau hauv nyias slices.
(3) Cov qauv tuaj yeem muab txhais thiab tig rau hauv peb qhov loj me hauv cov qauv chamber, yog li nws tuaj yeem pom los ntawm ntau lub kaum ntse ntse.
(4) Qhov tob ntawm daim teb yog loj, thiab cov duab yog nplua nuj nyob rau hauv peb-dimensional kev nkag siab. Qhov tob ntawm daim teb ntawm scanning electron microscopy yog ob peb puas lub sij hawm loj dua li ntawm optical microscopy thiab ob peb kaum lub sij hawm loj dua li ntawm kis electron microscopy.
(5) Lub magnification ntau ntawm cov duab yog dav, thiab cov kev daws teeb meem kuj kuj siab. Nws tuaj yeem nthuav tawm los ntawm kaum mus rau ntau pua txhiab zaus, thiab qhov tseem ceeb suav nrog ntau qhov kev nthuav dav los ntawm lub iav tsom iav, kho qhov muag tsom mus rau kev xa hluav taws xob microscope. Qhov kev daws teeb meem yog nruab nrab ntawm optical microscopy thiab kis tau tus mob electron microscopy, ncav cuag li 3nm.
(6) Qhov kev puas tsuaj thiab kev kis kab mob ntawm cov qauv los ntawm cov kab hluav taws xob yog qhov me me. (7) Thaum soj ntsuam cov morphology, lwm cov cim qhia tawm los ntawm cov qauv kuj tuaj yeem siv rau kev txheeb xyuas thaj tsam micro.
