Dab tsi yog qhov theoretical limiting magnification ntawm optical microscope?
Qhov kev daws teeb meem ntawm optical microscope yog hais txog 0.2 micrometers, uas sib haum mus rau qhov loj ntawm 1500 txog 2000 zaug; Txhawm rau kom ua tiav qhov loj dua, siv lub tshuab hluav taws xob hluav taws xob lossis tunneling scanning microscope.
Magnifying lo ntsiab muag tuaj yeem tsom lub teeb kom ua tiav qhov kev nthuav dav, siv kev sib txuas ntawm cov lo ntsiab muag kom tau txais optical microscopes; qhov kev txwv ntawm optical microscopes yog txwv los ntawm wavelength, thiab tsis muaj kev nthuav dav tsis tau.
Feem ntau hais lus, qhov kev daws teeb meem ntawm optical microscope nrog taag wavelength yog ib nrab ntawm lub wavelength ntawm lub teeb, thiab lub wavelength ntawm pom lub teeb nyob nruab nrab ntawm 4 00 ~ 760nm, yog li qhov kev daws teeb meem txwv ntawm optical microscope yog 200nm (0.2 micron). Cov khoom me dua 0.2 microns yuav tsis raug daws los ntawm lub tshuab ntsuas qhov muag, ib yam li qhov kev daws teeb meem ntawm tib neeg txhais tes tsis tuaj yeem tshaj qhov deb me me ntawm tactile hlwb.
Thaum magnification yog cov ntsiab lus, txhais raws li qhov piv ntawm qhov loj ntawm ib yam khoom pom los ntawm tib neeg lub qhov muag mus rau nws qhov loj me ntawm qhov deb ntawm 25cm hauv qhov pom, qhov 0.2 micron daws teeb meem ntawm lub tshuab ntsuas qhov muag yog sib npaug ntawm qhov loj ntawm 1500 mus rau 2000 zaug, uas yog txaus tso cai rau peb pom cov qauv ntawm nruab nrab ntawm tes kom meej meej.
Yog hais tias peb siv electromagnetic tsis nrog luv wavelengths, peb muaj peev xwm ua tau ntau dua magnification, tab sis qhov no yog dhau lub wavelength ntau ntawm pom lub teeb; Xyoo 1931, tus kws tshawb fawb Askiv Askiv Luska tau tsim lub tshuab ntsuas hluav taws xob, raws li lub hauv paus ntsiab lus ntawm nthwv dej-particle dual, lub teeb hluav taws xob muaj lub voj voog luv luv ntawm De Bruyne yoj, yog li nws tuaj yeem ua tiav qhov kev daws teeb meem me dua.
Qhov nrawm nrawm ntawm lub tshuab hluav taws xob thiab nws tus kheej lub wavelength sib raug rau, thaum qhov hluav taws xob hauv 100 kilovolts, lub wavelength ntawm electron beam yog li 0.004nm (qhov kev daws teeb meem tsuas yog ncav cuag 0.2nm) , kuj tseem me dua li lub wavelength ntawm pom lub teeb, yog li qhov kev daws teeb meem ntawm lub tshuab ntsuas hluav taws xob muaj ntau tshaj li qhov kho qhov muag lub tshuab tsom, qhov loj tuaj yeem paub qhov kev nthuav dav ntawm 3 lab lub sijhawm, tuaj yeem paub qhov txawv ntawm tus kab mob, mitochondria, DNA thiab lwm yam khoom me me.
