Dab tsi yog qhov optical system ntawm fluorescence microscope?
Nyob rau hauv lub tshuab microscope, vim yog cov yam ntxwv kho qhov muag ntawm qee yam khoom, lub tshuab ntsuas lub ntsej muag zoo tib yam tsis tuaj yeem pom lawv cov qauv sab hauv kom meej, tab sis lawv muaj lwm yam ntxwv. Piv txwv li, qee yam khoom hauv hlwb, xws li chlorophyll, tuaj yeem fluoresce tom qab irradiated los ntawm ultraviolet rays; Txawm hais tias qee yam khoom lawv tus kheej tsis tuaj yeem fluoresce, lawv tuaj yeem fluoresce tom qab dyeing nrog fluorescent dyes lossis fluorescent antibodies tom qab irradiated nrog ultraviolet lub teeb. Siv cov yam ntxwv kho qhov muag ntawm cov khoom no, cov khoom siv microdisplay, uas yog lub tshuab tsom fluorescence, tau tsim. Fluorescence microscopy yog ib qho cuab yeej rau kev tshawb fawb zoo thiab ntau yam ntawm cov khoom no. Lub ntsiab txhais meej ntawm lub tshuab tsom iav fluorescence yog hais txog lub tshuab tsom iav uas xaiv ib lub wavelength ntawm excitation lub teeb tawm los ntawm lub teeb siab mercury los yog lub teeb pom kev zoo sib xws rau excite tej yam khoom uas sau nrog fluorescent dyes nyob rau hauv lub hlwb kom emit fluorescence, thiab soj ntsuam cov kev faib tawm ntawm ib feem ntawm lub cell. Semi-quantitative ntsuam xyuas tuaj yeem ua tau. Fluorescence microscopy yog ib qho ntawm cov cuab yeej rau kev tshawb fawb zoo thiab ntau yam ntawm cov khoom no.
Kev hloov kho ntawm lub tshuab hluav taws xob teeb pom kev kho qhov muag kho qhov muag yog ua kom pom kev pom ntawm lub tshuab tsom iav tau txais cov teeb pom kev zoo thiab txaus illumination. Thaum lub tshuab ntsuas ntsuas thawj zaug thiab debugged, lub teeb pom kev kho qhov muag yuav tsum tau kho kom zoo. Qhov no yog ib qho tseem ceeb thiab qhov tseem ceeb tshaj plaws uas yuav tsum tau siv lub tshuab tsom iav kom raug thiab tau txais cov txiaj ntsig raug thiab txhim khu kev qha. Tsis tas li ntawd, kev paub zoo txog kev hloov pauv ntawm lub teeb pom kev ntawm lub teeb pom kev zoo yog ib kauj ruam tsim nyog tom qab hloov lub teeb qhov muag thaum lub sij hawm siv lub tshuab tsom, thiab nws tseem yog ib qho tsim nyog txhais tau tias txhawm rau txheeb xyuas qhov ua tau zoo ntawm lub tshuab tsom iav ib ntus hauv ib hnub. siv. Fluorescence microscopes txawv ntawm cov tshuab microscopes zoo li hauv qab no:
1. Txoj kev illumination feem ntau yog episcopic, uas yog, lub teeb qhov chaw yog projected ntawm tus qauv los ntawm lub hom phiaj lens;
2. Lub teeb ci yog ultraviolet lub teeb, lub wavelength yog luv dua, thiab qhov kev daws teeb meem yog siab dua li ntawm cov microscopes zoo tib yam;
3. Muaj ob lub lim dej tshwj xeeb, ib qho nyob rau pem hauv ntej ntawm lub teeb ci yog siv los lim tawm lub teeb pom kev, thiab ib qho nruab nrab ntawm lub qhov muag thiab lub hom phiaj lens yog siv los lim tawm ultraviolet rays los tiv thaiv tib neeg lub qhov muag.
Fluorescence microscope kuj yog ib yam ntawm optical microscope, qhov sib txawv tseem ceeb yog qhov excitation wavelength ntawm ob qhov sib txawv. Qhov no txiav txim siab qhov sib txawv ntawm fluorescence microscope thiab lub tshuab kho qhov muag zoo tib yam ntawm cov qauv thiab kev siv.
Fluorescence microscopy yog ib qho cuab yeej tseem ceeb hauv immunofluorescent cytochemistry. Nws yog tsim los ntawm cov khoom tseem ceeb xws li lub teeb ci, lim phaj system thiab optical system. Nws yog siv ib lub teeb ntawm lub teeb kom zoo siab rau cov qauv kom tawm fluorescence, thiab saib xyuas cov duab fluorescence ntawm cov qauv los ntawm kev nthuav dav lub hom phiaj lens thiab eyepiece system. Nws yog dav siv hauv kev tshawb fawb txog biology thiab tshuaj.
