Dab tsi yog qhov txawv ntawm inverted fluorescence microscopy thiab laser confocal microscopy
Laser confocal microscope yog ib txheej ntawm kev soj ntsuam, tsom xam, thiab tso zis tshuab uas siv laser raws li lub teeb qhov chaw, conjugate tsom lub hauv paus ntsiab lus thiab ntaus ntawv nyob rau hauv lub hauv paus ntawm ib txwm optical microscope, thiab digital image processing ntawm cov khoom pom siv lub computer. Lub tshuab tseem ceeb muaj xws li laser qhov chaw teeb pom kev zoo, tsis siv neeg microscopes, scanning modules (xws li confocal optical path channels thiab pinholes, scanning mirrors, detectors), digital signal processors, computers, thiab image output devices (displays, color printers). Los ntawm kev siv lub laser scanning confocal microscope, nws muaj peev xwm ua tomography thiab cov duab ntawm cov qauv pom. Yog li ntawd, nws muaj peev xwm soj ntsuam thiab txheeb xyuas qhov peb-dimensional spatial qauv ntawm cov hlwb yam tsis muaj kev puas tsuaj.
Nyob rau tib lub sijhawm, laser scanning confocal microscopy kuj yog ib qho cuab yeej muaj zog rau kev soj ntsuam ntawm cov hlwb nyob, ntau yam tshuaj tiv thaiv kab mob, thiab ion fluorescence labeling. Nws txheeb xyuas qhov tseeb ntawm cov spectrum thiab paub qhov txawv ntawm cov cim los ntawm cov ntawv sib txawv nrog cov emission spectrum sib tshooj.
Qhov tseem ceeb tshaj plaws yog tias rau ntau cov xim fluorescence staining, nws tuaj yeem tshem tawm tag nrho cov kev cuam tshuam ntawm fluorescence crosstalk, thaum txo qhov poob ntawm cov qauv fluorescence teeb liab. Cov no yog txhua yam uas iav zoo tib yam ua tsis tau.
Peb qhov sib txawv
1. Fluorescence microscope: Fluorescence microscope yog ib qho cuab yeej siv hauv immunofluorescence cytochemistry. Nws yog tsim los ntawm cov khoom tseem ceeb xws li lub teeb ci, lim phaj system, thiab kho qhov muag. Nws yog siv ib lub teeb ntawm lub teeb kom zoo siab rau cov qauv thiab emit fluorescence, thiab saib xyuas cov duab fluorescence ntawm cov qauv los ntawm lub hom phiaj thiab qhov muag pom qhov system amplification.
2. Laser confocal microscopy: Laser scanning confocal microscopy thev naus laus zis tau siv rau hauv kev tshawb fawb ntawm cell morphology localization, peb-dimensional recombination, dynamic hloov txheej txheem, thiab muab cov tswv yim tshawb fawb txoj kev xws li kev ntsuas fluorescence ntau npaum li cas thiab cov duab tsom xam ntau npaum li cas. Ua ke nrog lwm yam biotechnology, nws tau dav siv nyob rau hauv molecular cell biology teb xws li morphology, physiology, immunology, noob caj noob ces, thiab lwm yam.
