Dab tsi yog qhov txawv ntawm fluorescence microscope thiab inverted microscope
Hauv kab lis kev cai ntawm tes thiab lwm yam kev sim derivative, lub tshuab tsom iav yog ib qho cuab yeej tseem ceeb heev. Tam sim no, muaj ntau hom microscopes nyob rau hauv kev ua lag luam. Nws yog ib qho nyuaj rau xaiv lub tshuab tsom iav uas ua tau raws li qhov xav tau thiab siv tau. Cov hauv qab no yog kev qhia txog cov hauv paus ntsiab lus ntawm inverted microscopes thiab fluorescence microscopes, yog li koj tuaj yeem xaiv tau yooj yim.
Qhov sib xyaw ua ke ntawm lub tshuab tsom iav inverted yog tib yam li ntawm lub tshuab tsom iav zoo tib yam, feem ntau suav nrog peb ntu: cov khoom siv, qhov teeb pom kev zoo, thiab qhov muag pom.
Qhov sib xyaw ua ke ntawm lub tshuab ntsuas inverted yog tib yam li ntawm lub tshuab ntsuas lub ntsej muag zoo tib yam, tshwj tsis yog tias lub hom phiaj lens thiab lub teeb pom kev zoo tau thim rov qab, qhov qub yog nyob rau hauv theem, thiab tom kawg yog saum theem.
Cov qauv zoo li no tuaj yeem nthuav dav qhov kev ncua deb ntawm lub teeb pom kev zoo thiab cov theem, uas yooj yim rau kev tso cov khoom tuab kom pom, xws li kab lis kev cai tais diav thiab cell kab lis kev cai fwj (tau kawg, iav slides, thiab lwm yam.) , thiab nyob rau tib lub sij hawm, qhov kev ncua deb ntawm lub hom phiaj lens thiab cov khoom Qhov kev ua hauj lwm deb ntawm lawv tsis tas yuav loj heev.
Inverted microscopes yog siv rau kev soj ntsuam cov kab mob, cov hlwb, cov kab mob, cov kab mob hauv cov ntaub so ntswg, tshem tawm, cov quav, thiab lwm yam hauv cov chaw kho mob thiab kev noj qab haus huv, cov tsev kawm qib siab, thiab cov koom haum tshawb fawb. Nws tuaj yeem soj ntsuam cov txheej txheem ntawm kev loj hlob thiab kev faib tawm ntawm cov hlwb, cov kab mob, thiab lwm yam hauv cov kab lis kev cai nruab nrab, thiab tuaj yeem nqa cov duab ntawm txhua daim ntawv hauv cov txheej txheem.
Nws yog dav siv hauv cytology, parasitology, oncology, immunology, genetic engineering, industrial microbiology, botany thiab lwm yam.
Fluorescence microscopy yog siv los kawm txog kev nqus thiab kev thauj mus los ntawm cov tshuaj hauv cov hlwb, kev faib tawm thiab thaj chaw ntawm cov tshuaj lom neeg, thiab lwm yam.
Rau cov khoom nyob rau hauv kev soj ntsuam, muaj ob txoj kev los tsim fluorescence: autofluorescence, uas emits fluorescence ncaj qha tom qab irradiated nrog ultraviolet lub teeb;
Qee cov tshuaj hauv cov hlwb, xws li chlorophyll, tsim autofluorescence tom qab raug irradiated los ntawm ultraviolet rays; Txawm hais tias qee yam khoom lawv tus kheej tsis tuaj yeem fluoresce, lawv kuj tuaj yeem tawm cov fluorescence thib ob tom qab stained nrog fluorescent dyes lossis fluorescent antibodies tom qab raug irradiated los ntawm ultraviolet rays.
Fluorescence microscope siv lub teeb pom kev zoo nrog kev ua haujlwm siab luminous kom tso tawm lub teeb ntawm qee qhov wavelength (ultraviolet teeb 365nm lossis ntshav xiav lub teeb 420nm) los ntawm lub lim dej ua lub teeb pom kev zoo, thiab tom qab zoo siab cov tshuaj fluorescent hauv cov qauv kom tawm fluorescence ntawm ntau yam. xim, tom qab ntawd Kev soj ntsuam yog ua los ntawm kev nthuav dav ntawm lub hom phiaj lens thiab eyepiece.
Nyob rau hauv txoj kev no, nyob rau hauv ib tug muaj zog sib piv keeb kwm yav dhau, txawm yog hais tias tus fluorescence yog heev qaug zog, nws yog ib qho yooj yim los txheeb xyuas thiab muaj siab rhiab heev. Nws yog tsuas yog siv rau kev tshawb fawb ntawm cov qauv ntawm tes thiab kev ua haujlwm thiab tshuaj lom neeg muaj pes tsawg leeg.
