Koj paub dab tsi txog qhov sib txawv ntawm qhov sib txawv microscopy (DIC)?
Lub peev xwm los saib thiab ntsuas cov kev hloov pauv me me, zoo ib yam li lub tshuab ntsuas theem, muab cov xim tsis muaj xim thiab pob tshab hloov pauv hauv lub teeb, tsaus thiab xim, yog li txhim kho qhov sib txawv. Polarizing thiab cuam tshuam cov khoom yog haum rau cov qauv yooj yim ntawm lub tshuab kho qhov muag zoo tib yam, nrog rau 360 degree rotating theem. Nws nyob rau hauv lem ua rau siv lub hauv paus ntsiab lus ntawm kev cuam tshuam ntawm lub teeb polarized. Raws li pom hauv daim duab 7, lub lens polarizing thiab beam-disintegrating prism yog muab tso rau saum lub teeb. Lub teeb linearly polarized los ntawm lub lens polarizing hla lub beam decomposition prism, thiab ces splits rau hauv ob linearly polarized teeb rays uas vibrate perpendicular rau ib leeg. Ob lub teeb rays yog refracted los ntawm concentrator thiab coj mus rau tus qauv. Vim muaj qhov sib txawv refractive indices ntawm txhua qhov taw tes hauv cov qauv, cov theem ntawm qee lub teeb tsis hloov pauv thiab hloov mus tom qab vim muaj kev cuam tshuam. Ob lub rays ntawm lub teeb hla dhau lub hom phiaj lo ntsiab muag thiab tau ua ke los ntawm ib pawg ntawm cov kab hluav taws xob-disintegrating prisms thiab cuam tshuam los ntawm daim iav tsis pom kev. Txhua lub ntsiab lus ntawm daim duab kawg yog ib daim duab hybrid uas muaj ob daim duab sib tshooj ntawm tib lub ntsiab lus ntawm cov khoom, ua rau nws pom ntawm qhov muag liab qab.
Qhov sib txawv ntawm qhov sib txawv Microscope kuj tuaj yeem soj ntsuam cov khoom tsis muaj xim thiab pob tshab uas tsis tuaj yeem pom hauv qhov pom kev ci ntsa iab zoo tib yam, thiab tuaj yeem soj ntsuam cov hlwb, ** thiab lwm yam kab mob nyob, thiab cov duab yog peb-dimensional, ntau cov ncauj lus kom ntxaws thiab muaj tseeb dua li cov daim duab ntawm theem microscope. Nws tuaj yeem siv rau kev kawm paub ntau ntxiv ntawm ntau qhov chaw ntawm cov cell nyob. Yog tias lub teeb dawb siv rau kev teeb pom kev zoo, cov theem sib txawv tau qhia ntau yam xim, thiab cov xim yuav hloov thaum lub sijhawm tig. Monochromatic illumination ua qhov sib txawv ntawm lub teeb thiab tsaus, thiab ntau yam khoom qhia qhov sib txawv. Differential Interference Difference (DID) microscopes kuj tseem siv tau los ua qhov sib npaug ultra-micro-optical sib npaug rau kwv yees cov khoom qhuav nrog qhov tseeb qhov tseeb me me li 1 x -14 g. Lub microscope kuj tseem siv tau los ua qhov sib npaug ultra-micro-optical nrog rau qib siab ntawm qhov tseeb. Raws li qhov concentration ntawm cov khoom uas muaj nyob rau hauv ib lub cell nce los ntawm ib xees, nws refractive index nce los ntawm 0.0018. Lub refractive index ntawm txhua theem ntawm ib lub xovtooj ntawm tes yuav kwv yees los ntawm qhov sib txawv ntawm hom ntawm nws thiab thaj tsam ntawm kev txaus siab (lub cheeb tsam ncua kev kawm ntawv), thiab yog li qhov hnyav qhuav ntawm qee yam ntawm ib lub xovtooj tuaj yeem suav ntxiv.
