Siv Fluorescence Microscopy: Cov Txheej Txheem thiab Kev Txawj Ntse
Fluorescence Microscope Technical Parameters 1. Wide-angle eyepiece 2. Achromatic objective lens 3. Four-hole objective lens converter 4. Epi-fluorescent device Blue (B) Green (G) excitation system 100W mercury teeb 5. Coaxial ntxhib thiab nplua ua kom pom tseeb kev hloov kho mechanism: kev hloov kho kom pom tseeb ntau yam: 15mm Micro-txav daim phiaj tus nqi: 0.002mm 6. Ob txheej txheej tshuab tshuab Longitudinal txav ntau: 70mm Lateral txav ntau: 50mm
Siv txoj kev txawj
(1) iav swb
Lub thickness ntawm cov iav swb yuav tsum nyob nruab nrab ntawm 0.8 thiab 1.2mm. Ib tug swb uas tuab heev yuav nqus tau ntau lub teeb ntawm ib sab tes, thiab ntawm qhov tod tes, lub teeb excitation tsis tuaj yeem tsom rau ntawm cov qauv. Cov zawv zawg yuav tsum du, sib xws hauv tuab, thiab tsis muaj qhov pom tseeb autofluorescence. Qee zaum quartz iav slides yog siv.
(2) Npog iav
Lub thickness ntawm lub hau iav yog li ntawm 0.17mm, du. Txhawm rau ntxiv dag zog rau lub teeb pom kev zoo, ib qho kev cuam tshuam npog iav tuaj yeem siv tau, uas yog lub npog tshwj xeeb iav coated nrog ob peb txheej ntawm cov tshuaj (xws li magnesium fluoride) uas muaj kev cuam tshuam sib txawv ntawm lub teeb ntawm qhov sib txawv wavelengths, uas tuaj yeem ua rau lub teeb pom kev zoo. fluorescence mus smoothly. Lub teeb zoo siab yog dhau los ntawm thiab cuam tshuam, thiab qhov pom kev zoo siab lub teeb zoo siab rau cov qauv.
(3) Cov qauv
Cov ntaub so ntswg los yog lwm cov qauv yuav tsum tsis txhob tuab heev. Yog hais tias nws yog tuab heev, feem ntau ntawm cov excitation teeb yuav tau noj nyob rau hauv qis ib feem ntawm cov qauv, thaum lub sab sauv ncaj qha soj ntsuam los ntawm lub hom phiaj lens yuav tsis zoo siab heev. Tsis tas li ntawd, cell overlapping lossis impurity npog, cuam tshuam rau kev txiav txim.
(4) Mounting tus neeg sawv cev
Glycerin feem ntau yog siv los ua tus neeg sawv cev, uas yuav tsum tsis muaj autofluorescence, tsis muaj xim thiab pob tshab, thiab qhov ci ntawm cov fluorescence yog ci dua ntawm pH 8.5-9.5, thiab nws tsis yooj yim kom ploj mus sai. Yog li, qhov sib npaug ntawm glycerol thiab 0.5mol / l carbonate buffer tov nrog pH ntawm 9.0 rau 9.5 feem ntau yog siv los ua tus neeg sawv cev.
(5) iav roj
Feem ntau, thaum soj ntsuam cov qauv nrog qhov tsaus ntuj nti fluorescence microscopes thiab roj immersion lo ntsiab muag, yuav tsum tau siv roj immersion. Nws yog qhov zoo tshaj plaws los siv cov roj tshwj xeeb uas tsis muaj fluorescent immersion. Cov glycerin saum toj no kuj siv tau los hloov, thiab cov kua paraffin kuj tseem siv tau, tab sis qhov ntsuas qhov ntsuas qis qis, uas muaj kev cuam tshuam me ntsis ntawm cov duab zoo. Kev npam.
