Lub superiority ntawm laser confocal microscopy hauv biology
Piv nrog rau cov tshuaj optical microscope, laser confocal microscope muaj kev daws teeb meem ntau dua, ntau yam fluorescence tuaj yeem pom tib lub sijhawm thiab tuaj yeem tsim cov duab peb sab thiab lwm yam zoo, hauv kev soj ntsuam cov qauv lom neeg, laser confocal microscope muaj cov txiaj ntsig hauv qab no. :
1, Kev soj ntsuam tas li ntawm cov ntaub so ntswg ntawm lub cev los yog cov xov ntawm tes tuaj yeem tau txais cov duab zoo peb sab ntawm cytoskeleton, chromosomes, organelles thiab cell membrane systems.
2, Nws tuaj yeem tau txais qhov sib txawv siab dua thiab cov duab daws teeb meem siab dua li cov fluorescence microscope zoo tib yam, lub sijhawm no, nws muaj kev nkag siab zoo thiab kev tiv thaiv zoo heev.
3, Multi-dimensional duab nrhiav tau. Xws li 7-dim duab (XYZaλIt): xyt , xzt thiab xt scanning, lub sij hawm series scanning, rotational scanning, regional scanning, spectral scanning, thaum uas yooj yim rau cov duab ua.
4, Intracellular ion fluorescence labelling. Ib qho los yog ntau daim ntawv lo, nrhiav kom pom cov ions intracellular xws li PH thiab sodium, calcium, magnesium thiab lwm yam ions concentration piv ntsuas thiab dynamic hloov.
5, Fluorescence labelling. Kev sojntsuam sau npe nyob rau hauv lub hlwb los yog hlais cov qauv ntawm cov kabmob muaj sia, cov cim cim, cov tshuaj, cov tshuaj tiv thaiv, cov receptors lossis ligands, nucleic acids thiab lwm yam kev soj ntsuam; tuaj yeem nyob hauv tib qho qauv tib lub sijhawm rau ntau yam tshuaj uas tau sau tseg thiab pom nyob rau tib lub sijhawm.
6, tsis muaj kev puas tsuaj rau kev tshawb pom ntawm tes, muaj kev nyab xeeb, txhim khu kev qha thiab zoo heev repeatability; cov ntaub ntawv dluab tuaj yeem tso tawm hauv lub sijhawm lossis kev cia ntev ntev.
