Lub luag haujlwm ntawm txhua lub lim hauv fluorescence microscopy
Qhov teeb meem tseem ceeb tshaj plaws nrog fluorescence microscopy yog kev sib xyaw ntawm cov ntxaij lim dej, thiab ob qho tib si lub lim dej thiab cov ntaub thaiv npog yuav tsum tsim nyog rau lub cev fluorescent pom thiab sib txuam nrog ib leeg. Rau qee lub hom phiaj tshwj xeeb, yog tias muaj qhov yuam kev hauv kev xaiv lub lim, nws yuav ua rau muaj kev nkag siab yuam kev thiab txawm tias tsis raug. Kev ua tiav ntawm fluorescence microscopy thev naus laus zis thiab kev siv cov lim dej zoo thiab cov pab pawg lim ingenious yog sib cais tsis tau. Muaj ntau ntau hom lim rau fluorescence microscopy.
1. Thaum tshav kub kub absorbing lim
Thaum tshav kub kub absorbing lim yog ib tug tsim nyog lim los tiv thaiv thermal tawg nyob rau hauv lub teeb qhov chaw spectrum los ntawm kev puas tsuaj rau cov kho qhov muag pab pawg neeg. Thaum ntxov Advanced microscopes tau nruab nrog lub nruas zoo li lub raj mis uas tuaj yeem tuav cov dej distilled li cov khoom siv cua sov. Niaj hnub nimno loj-scale tshawb fawb microscopes yog nruab nrog iav kub absorbing lim nyob rau hauv lub teeb qhov chaw lub teeb thawv los yog chambers, uas yog pob tshab, me ntsis daj iav los xij. Leitz's KG1 (2 hli) thiab BG38 (4 hli) cov ntxaij lim dej tuaj yeem kis tau yuav luag txhua qhov ultraviolet thiab pom lub teeb pom kev zoo, ua rau lawv lim dej nrog lub teeb flux txog li 98%. Nws tsuas yog xaiv absorbs infrared photothermal kab hluav taws xob.
2. Excitation lim
Excitation filters tuaj yeem xaiv nqus cov kab ntev wavelength spectral thiab tsuas yog xa cov teeb ci ultraviolet. Cov lim rau ntshav, xiav, thiab lub teeb ntsuab yog cov lim dej kom zoo. Zeiss, Reichert, Olympus B-series, G-series, BG series, Shott UG series, Opton H, G, BP series, Leitz BP series thiab lwm cov lim dej yog tag nrho cov lim dej.
3. Thaiv lim
Blocking filter yog ib lub lim uas xaiv absorbs luv wavelength spectral kab thiab infrared hluav taws xob, tso cai rau tus neeg soj ntsuam pom cov fluorescence emission los ntawm cov khoom raug kuaj thaum tiv thaiv lub cornea los ntawm UV puas.
4. Xim lub teeb cais lim
Xim lub teeb sib cais lim yog lub lim uas qhia txog lub teeb pom kev zoo mus rau qhov khoom raug sim, ua rau cov khoom tawm fluorescence, thiab tom qab ntawd xa cov fluorescence mus rau qhov muag. Hom lim no tsuas yog siv tau rau hauv cov teeb pom kev poob qis, thaum kis lub teeb fluorescence microscopy tsis tas yuav muaj xim sib cais.
5. cuam tshuam lim
Interference filter yog ib hom kev ua haujlwm siab excitation lim. Nws yog ib lub lim ua los ntawm stacking ob peb nyias hlau zaj duab xis ntawm ob daim iav polished. Lub refractive index ntawm txhua nyias hlau zaj duab xis yog sib txawv, yog li cov spectral kab ntawm txawv wavelengths ntawm lub illumination lub teeb qhov chaw yog rov qab xav nyob rau hauv ib tug hlau zaj duab xis, ua rau ib co wavelengths ntawm spectral kab tshem tawm vim kev puas tsuaj cuam tshuam, thaum lwm tus muaj zog los ntawm. additive cuam tshuam thiab kis tau los ntawm. Qhov no ua rau lub lim nrog qhov nqaim kis tau tus mob, ib nrab qhov siab dav ntawm tsuas yog 6 ~ 20nm, thiab kev xa tawm ntawm 60% ~ 70%.
