Cov hauv paus ntsiab lus thiab cov qauv tsim ntawm fluorescence microscope
Fluorescence microscopy siv lub ntsiab lus nrog kev ua haujlwm siab luminous kom tso tawm lub teeb ntawm qee qhov wavelength (xws li lub teeb ultraviolet 3650 hauv lossis ntshav xiav lub teeb 4200 hauv) los ntawm lub lim dej ua lub teeb ci kom zoo siab rau cov tshuaj fluorescent hauv cov qauv kom tawm fluorescence ntawm Ntau xim Tom qab ntawd, saib los ntawm kev nthuav dav ntawm lub hom phiaj lens thiab eyepiece. Nyob rau hauv txoj kev no, nyob rau hauv ib tug muaj zog sib piv keeb kwm yav dhau, txawm yog hais tias tus fluorescence yog heev qaug zog, nws yog ib qho yooj yim los txheeb xyuas thiab muaj siab rhiab heev. Nws yog tsuas yog siv rau kev tshawb fawb ntawm cov qauv ntawm tes thiab kev ua haujlwm thiab tshuaj lom neeg muaj pes tsawg leeg. Cov qauv yooj yim ntawm fluorescence microscope yog tsim los ntawm ib lub tshuab kho qhov muag zoo tib yam ntxiv rau qee yam khoom siv (xws li lub teeb fluorescent, lub tshuab lim dej, ob lub xim kab sib cais thiab cov lim lim, thiab lwm yam). Fluorescent teeb qhov chaw-feem ntau yog siv ultra-siab siab mercury teeb (50-200W), uas muaj peev xwm emit lub teeb ntawm ntau lub wavelengths, tab sis txhua yam fluorescent tshuaj muaj ib tug excitation wavelength uas ua rau cov fluorescence muaj zog tshaj, yog li ib tug excitation lim (Feem ntau, muaj ultraviolet, ntshav, xiav thiab ntsuab excitation lim), uas tsuas yog tso cai rau lub teeb excitation ntawm ib co wavelength los ntawm thiab irradiate tus qauv, thaum absorbing lwm lub teeb. Tom qab txhua yam khoom yog irradiated los ntawm excitation lub teeb, nws emits pom fluorescence nrog ib tug ntev wavelength tshaj lub irradiation wavelength nyob rau hauv ib tug luv luv lub sij hawm. Fluorescence yog qhov tshwj xeeb thiab feem ntau tsis muaj zog dua li lub teeb pom kev zoo. Txhawm rau saib xyuas cov fluorescence tshwj xeeb, thaiv (lossis kev tawm tsam) yuav tsum tau ntxiv tom qab lub hom phiaj lens thiab siv ua ke nrog nws.
Qhov txawv ntawm fluorescence microscope thiab dog dig microscope
1. Txoj kev illumination feem ntau yog episcopic, uas yog, lub teeb qhov chaw yog projected ntawm tus qauv los ntawm lub hom phiaj lens;
2. Lub teeb ci yog ultraviolet lub teeb, lub wavelength yog luv dua, thiab qhov kev daws teeb meem yog siab dua li ntawm cov microscopes zoo tib yam;
3. Muaj ob lub lim dej tshwj xeeb, ib qho nyob rau pem hauv ntej ntawm lub teeb ci yog siv los lim tawm lub teeb pom kev, thiab ib qho nruab nrab ntawm lub qhov muag thiab lub hom phiaj lens yog siv los lim tawm ultraviolet rays los tiv thaiv tib neeg lub qhov muag.
Fluorescence microscope kuj yog ib yam ntawm optical microscope, qhov sib txawv tseem ceeb yog qhov excitation wavelength ntawm ob qhov sib txawv. Qhov no txiav txim siab qhov sib txawv ntawm fluorescence microscope thiab lub tshuab kho qhov muag zoo tib yam ntawm cov qauv thiab kev siv.
Fluorescence microscopy yog ib qho cuab yeej tseem ceeb hauv immunofluorescent cytochemistry. Nws yog tsim los ntawm cov khoom tseem ceeb xws li lub teeb ci, lim phaj system thiab optical system. Nws yog siv ib lub teeb ntawm lub teeb kom zoo siab rau cov qauv kom tawm fluorescence, thiab saib xyuas cov duab fluorescence ntawm cov qauv los ntawm kev nthuav dav lub hom phiaj lens thiab eyepiece system.
