Qhov sib txawv ntawm ob-photon microscopy thiab laser confocal microscopy
Laser confocal microscope yog ib txheej ntawm kev soj ntsuam, tsom xam, thiab tso zis tshuab uas siv laser raws li lub teeb qhov chaw, conjugate tsom lub hauv paus ntsiab lus thiab ntaus ntawv nyob rau hauv lub hauv paus ntawm ib txwm optical microscope, thiab digital image processing ntawm cov khoom pom siv lub computer. Lub tshuab tseem ceeb muaj xws li laser qhov chaw teeb pom kev zoo, tsis siv neeg microscopes, scanning modules (xws li confocal optical path channels thiab pinholes, scanning mirrors, detectors), digital signal processors, computers, thiab image output devices (displays, color printers). Los ntawm kev siv lub laser scanning confocal microscope, nws muaj peev xwm ua tomography thiab cov duab ntawm cov qauv pom. Yog li ntawd, nws muaj peev xwm soj ntsuam thiab txheeb xyuas cov qauv peb-dimensional spatial ntawm cov hlwb yam tsis muaj kev puas tsuaj.
Nyob rau tib lub sijhawm, laser scanning confocal microscopy kuj yog ib qho cuab yeej muaj zog rau kev soj ntsuam cov hlwb nyob, ntau yam tshuaj tiv thaiv kab mob, thiab ion fluorescence labeling. Nws txheeb xyuas qhov tseeb ntawm cov spectrum thiab paub qhov txawv ntawm cov cim los ntawm cov ntawv sib txawv nrog cov emissions spectrum sib tshooj.
Qhov tseem ceeb tshaj plaws yog tias rau ntau cov xim fluorescence staining, nws tuaj yeem tshem tawm tag nrho cov kev cuam tshuam ntawm fluorescence crosstalk, thaum txo qhov poob ntawm cov qauv fluorescence teeb liab. Cov no yog txhua yam uas iav zoo tib yam ua tsis tau.
Lub focal ntev kev sib raug zoo ntawm lub hom phiaj tsom iav thiab lub qhov muag
Cov ntsiab cai txawv
1. Fluorescence microscope: Nws siv lub teeb ci ultraviolet los ua lub teeb ci los teeb pom cov khoom uas tau sim, ua rau nws tawm fluorescence, thiab tom qab ntawd saib cov duab thiab qhov chaw ntawm cov khoom hauv qab lub tshuab tsom.
2. Laser confocal microscope: Raws li lub hauv paus ntawm fluorescence microscopy imaging, ib tug laser scanning ntaus ntawv yog ntsia rau excite fluorescence probes siv ultraviolet los yog pom lub teeb.
Cov yam ntxwv sib txawv
1. Fluorescence microscope: siv los kawm txog kev nqus, kev thauj mus los, kev faib tawm, thiab chaw nyob ntawm cov khoom hauv hlwb. Qee yam khoom hauv hlwb, xws li chlorophyll, tuaj yeem tawm fluorescence tom qab raug ultraviolet hluav taws xob; Qee cov tshuaj lawv tus kheej yuav tsis tawm fluorescence, tab sis yog stained nrog fluorescent dyes lossis fluorescent antibodies, lawv tuaj yeem tawm fluorescence nyob rau hauv ultraviolet hluav taws xob.
2. Laser confocal microscope: Siv lub khoos phis tawj rau kev ua duab kom tau txais cov duab fluorescent ntawm sab hauv microstructure ntawm cov hlwb lossis cov ntaub so ntswg, thiab saib xyuas lub cev muaj zog xws li Ca2+, pH tus nqi, daim nyias nyias, thiab kev hloov pauv hauv cell morphology nyob rau theem subcellular.
