Qhov sib txawv ntawm fluorescence thiab kev ua kom haum
1, fluorescence tsom iav
1. Fluorescence microscope uses ultraviolet light as a light source to irradiate the object being inspected, causing it to emit fluorescence, and then observe the shape and position of the object under the microscope. Fluorescence microscopy yog siv los kawm qhov nqus, thauj, faib khoom, thiab cov tshuaj hauv zos hauv cov tshuaj. Qee cov tshuaj hauv cov qog, xws li chlorophyll, tuaj yeem fluoresce thaum raug rau ultraviolet hluav taws xob; Tseem muaj qee yam tshuaj uas tsis tuaj yeem tawm fluorescence lawv tus kheej, tab sis kuj tseem tuaj yeem tso cov tshuaj tsw qab lossis ua kom muaj cov tshuaj lom fluorescodies thiab irradiated nrog ultraviolet teeb. Fluorescence microscopy yog ib qho ntawm cov cuab yeej rau cov kev tshawb fawb thiab kev tshawb fawb kom pom tseeb ntawm cov tshuaj no.
2. Lub ntsiab cai ntawm fluorescence tsom iav:
(A) Qhov Chaw Ntsuas Lub Teeb: Lub hauv paus teeb pom kev ntawm ntau yoj yoj (los ntawm ultraviolet mus rau infrared).
(B) Kev zam lub teeb ci lim: Xa cov nthwv dej tshwj xeeb uas tuaj yeem ua rau fluorength ci, thaum thaiv lub teeb uas tsis muaj txiaj ntsig zoo rau cov fluorescence.
(C) Cov yam ntxwv fluorescent: feem ntau tsuas nrog cov xim fluorescent.
(D) Kev thaiv cov lim: nws xaiv tau transmits fluorescence los ntawm cov quav dev, thiab ib co wavelengths hauv fluorescence kuj tseem xaiv tau kis. Lub tsom iav tsom uas siv cov teeb ci ultraviolet ua lub teeb ci kom ua rau qhov chaw ua kom zoo nkauj tshwm sim fluorescence. Lub tshuab hluav taws xob hauv hluav taws xob tau sib sau ua ntej los ntawm Knorr thiab Haruster siv hluav taws xob hluav taws xob ceev dua tshiab hloov chaw teeb ci. Vim tias cov wavelength ntau ntawm cov hluav taws xob ntws piv rau lub teeb hluav taws xob tau mus txog 8}}}}}}}}}}}}}}}}}}}}}}}}}}}}}} Lub scanning Electron tsom iav, uas tau siv thawj zaug hauv xyoo 1963, tso cai rau tib neeg pom cov qauv me me ntawm cov khoom ntawm cov khoom.
3. Daim ntawv thov tau txais: Siv cov duab loj ntawm cov khoom me me. Feem ntau siv rau kev soj ntsuam ntawm biology, tshuaj, microscopic hais, thiab lwm yam.
