Qhov sib txawv ntawm lub tshuab ntsuas hluav taws xob, lub tshuab hluav taws xob atomic force microscope thiab scanning tunneling microscope
I. Cov yam ntxwv ntawm scanning electron microscope Piv nrog optical microscope thiab kis electron microscope, scanning electron microscope muaj cov yam ntxwv hauv qab no:
(i) muaj peev xwm saib xyuas cov qauv ntawm cov qauv saum npoo, qhov loj ntawm cov qauv tuaj yeem loj li 120mm × 80mm × 50mm.
(ii) Cov txheej txheem kev npaj ua qauv yog yooj yim, tsis tas yuav txiav rau hauv nyias slices.
(iii) Cov qauv tuaj yeem muab txhais thiab tig rau hauv peb qib ntawm qhov chaw hauv chav kuaj, kom cov qauv tuaj yeem pom los ntawm ntau lub kaum ntse ntse.
(iv) Qhov tob ntawm daim teb yog loj, thiab cov duab yog nplua nuj nyob rau hauv peb-dimensional kev nkag siab. Qhov tob ntawm thaj tsam ntawm SEM yog ntau pua lub sij hawm loj dua li ntawm optical microscope thiab kaum tawm lub sij hawm loj dua qhov kis tau tus mob electron microscope.
(E) daim duab magnification ntau yog dav, qhov kev daws teeb meem kuj tseem siab. Nws tuaj yeem nthuav tawm ntau lub sijhawm rau ntau pua txhiab zaus, nws pib suav nrog los ntawm cov iav tsom iav, kho qhov muag tsom iav kom txog thaum kis tau tus mob electron microscope magnification ntau yam. Kev daws teeb meem ntawm optical microscope thiab kis tau tus mob electron microscope, mus txog 3nm.
(vi) Kev puas tsuaj thiab paug ntawm cov qauv los ntawm cov kab hluav taws xob yog me me.
(vii) Thaum soj ntsuam cov morphology, lwm cov cim qhia tawm los ntawm cov qauv kuj tuaj yeem siv rau kev tsom xam cov cheeb tsam micro.
II-Atomic Force Microscope
Atomic Force Microscope (AFM), cov cuab yeej tshuaj ntsuam xyuas uas tuaj yeem siv los kawm cov qauv saum npoo ntawm cov khoom siv, suav nrog cov insulators. Nws tshawb xyuas cov qauv saum npoo thiab cov khoom ntawm cov khoom los ntawm kev kuaj xyuas qhov tsis muaj zog ntawm kev sib cuam tshuam sib cuam tshuam ntawm lub ntsej muag ntawm cov qauv uas yuav tsum tau sim thiab cov khoom me me ntawm lub zog-rhiab heev. Ib khub ntawm micro-cantilevers, uas tsis tshua muaj siab rau lub zog tsis muaj zog, yog tsau ntawm ib qho kawg, thiab ib rab koob me me ntawm lwm qhov kawg yog coj los ze rau tus qauv, uas yuav cuam tshuam nrog nws, thiab lub zog yuav ua rau lub cev muaj zog. micro-cantilevers kom deform lossis hloov lawv lub xeev ntawm kev txav. Thaum kuaj xyuas cov qauv, cov kev hloov no raug kuaj pom los ntawm cov sensors, thiab cov ntaub ntawv ntawm kev faib tawm ntawm lub zog tuaj yeem tau txais, yog li tau txais cov ntaub ntawv ntawm qhov chaw morphology thiab cov qauv nrog rau qhov roughness ntawm nanometer daws teeb meem.
AFM muaj ntau yam zoo dua scanning electron microscopy. Tsis zoo li electron microscopes, uas tuaj yeem muab cov duab ob sab xwb, AFM muab qhov tseeb peb-dimensional daim duab qhia chaw. Tsis tas li ntawd, AFM tsis xav tau kev kho tshwj xeeb ntawm cov qauv, xws li tooj liab lossis carbon plating, uas tuaj yeem ua rau cov qauv tsis zoo. Thib peb, thaum electron microscopes yuav tsum tau ua haujlwm nyob rau hauv lub tshuab nqus tsev siab, AFMs ua haujlwm zoo ntawm atmospheric siab thiab txawm nyob rau hauv cov kua ib puag ncig. Qhov no tuaj yeem siv los kawm txog cov macromolecules lom thiab txawm tias cov ntaub so ntswg nyob. AFM muaj kev siv dav dua li Scanning Tunneling Microscope (STM) vim nws lub peev xwm los soj ntsuam cov qauv tsis ua. Scanning force microscopes, uas tam sim no tau siv dav hauv kev tshawb fawb thiab kev lag luam, yog ua raws li atomic force microscopy.
Scanning Tunneling Microscope
① high-resolution scanning tunneling microscope nrog atomic-theem spatial daws teeb meem, nws kab rov tav spatial daws teeb meem ntawm l, ntsug daws teeb meem ntawm 0.1, ② scanning tunneling microscope yuav siv tau nyob rau hauv lub teb ntawm atomic force microscopy.
② scanning tunneling microscope tuaj yeem tshawb xyuas cov qauv saum npoo ntawm cov qauv, tuaj yeem kos duab peb sab.
③ Scanning tunneling microscope tuaj yeem soj ntsuam cov qauv ntawm cov khoom hauv lub tshuab nqus tsev, atmospheric siab, huab cua, thiab txawm tias hauv kev daws teeb meem. Txij li thaum tsis muaj hluav taws xob hluav taws xob hluav taws xob loj, tsis muaj kev puas tsuaj rau saum npoo (xws li hluav taws xob, thermal puas, thiab lwm yam), yog li nws muaj peev xwm kawm txog cov qauv ntawm biomolecules thiab qhov chaw ntawm cov cell membranes nyob hauv lub xeev physiological. , thiab cov qauv yuav tsis puas thiab nyob twj ywm.
④ Scanning tunneling microscope muaj lub scanning ceev ceev, luv cov ntaub ntawv tau lub sij hawm, thiab ceev imaging, uas ua rau nws muaj peev xwm mus nqa tawm kinetic kev tshawb fawb ntawm lub neej txheej txheem.
⑤ Nws tsis tas yuav muaj lub lens thiab me me, yog li qee tus neeg hu nws tias "lub hnab tshos lub koob yees duab".
