Qhov zoo ntawm laser confocal microscopy yog
Laser confocal microscope yog ib lub tshuab tshiab uas ua ke nrog laser technology, microscope technology, fluorescence technology, computer thiab image processing technology, precision mechanical technology, thiab lwm yam, uas yog ib tug tshiab technology ntawm high, meej thiab ntse cell tsom xam thiab engineering technology. Nws tau dhau los ua tiam tshiab ntawm cov cuab yeej tshawb fawb muaj zog hauv kev ua haujlwm ntawm morphology, molecular cell biology, neuroscience, pharmacology, noob caj noob ces thiab lwm yam.
Laser confocal microscope yog niaj hnub no * qhov zoo tshaj plaws optical microscope, nws lub ntsiab zoo yog:
1, laser raws li lub teeb pom kev zoo, nyob rau hauv qhov sib thooj fluorescent sojntsuam labelling, taw tes los ntawm point scanning ntawm tus qauv, txheej los ntawm txheej kom tau ob-dimensional optical cross-sectional dluab, nrog rau kev ua ntawm "cell CT", thiab los ntawm lub computer. Peb-dimensional reconstruction software txhawb nqa, kom tau txais cov duab peb-dimensional, thiab tuaj yeem tig ntawm lub kaum ntse ntse, kev soj ntsuam ntawm cov hlwb, cov ntaub so ntswg, peb-dimensional morphology thiab spatial kev sib raug zoo. Nws tuaj yeem tig ntawm txhua lub kaum sab xis los saib xyuas cov duab peb sab thiab spatial kev sib raug zoo ntawm cov hlwb thiab cov ntaub so ntswg;
2, Nws tuaj yeem soj ntsuam cov hlwb thiab cov ntaub so ntswg yam tsis muaj kev puas tsuaj, thiab ntsuas qhov ntsuas intracellular Ca ion concentration thiab pH tus nqi thiab lwm yam ntaub ntawv physiological ntawm cov cell nyob;
3, Nws tuaj yeem siv los ua "lub teeb riam" los ua kom tiav intracellular "surgery" ntawm cell membrane fluidity, intercellular kev sib txuas lus, cell fusion, cytoskeleton elasticity ntsuas. Cov cuab yeej no tuaj yeem ua tiav hauv qhov kev soj ntsuam ntau qhov kev soj ntsuam thiab ntsuas ntawm cov cell thiab cov ntaub so ntswg.
