Cov qauv thiab cov khoom tseem ceeb ntawm fluorescence microscope
Fluorescence microscopy yog ib qho cuab yeej tseem ceeb hauv immunofluorescent cytochemistry. Nws yog tsim los ntawm cov khoom tseem ceeb xws li lub teeb ci, lim phaj system thiab optical system. Nws yog siv ib lub teeb ntawm lub teeb kom zoo siab rau cov qauv kom tawm fluorescence, thiab saib xyuas cov duab fluorescence ntawm cov qauv los ntawm kev nthuav dav lub hom phiaj lens thiab eyepiece system.
Cov qauv thiab cov khoom tseem ceeb ntawm fluorescence microscope
(1) Lub teeb ci
Niaj hnub no, 200W ultra-siab siab mercury teeb feem ntau siv los ua lub teeb ci. Lawv yog ua los ntawm quartz iav, nrog ib tug spherical zoo nyob rau hauv nruab nrab, thiab ib co mercury yog sau rau hauv. Thaum lub sij hawm ua hauj lwm, cov paug tawm ntawm ob lub electrodes ua rau mercury evaporate, thiab huab cua siab nyob rau hauv lub pob nce sai heev. Thaum cov mercury tag nrho evaporated, nws tuaj yeem ncav cuag 50-70 tus qauv atmospheric siab, thiab cov txheej txheem no feem ntau yuav siv li ntawm 5-15 feeb. Lub luminescence ntawm ultra-siab siab mercury teeb yog tshwm sim los ntawm emission ntawm lub teeb quanta thaum lub sij hawm tas mus li dissociation thiab txo cov mercury molecules los ntawm cov paug ntawm cov electrodes. Nws emits muaj zog ultraviolet thiab xiav-violet lub teeb, uas yog txaus rau excite ntau yam fluorescent tshuaj, yog li nws yog dav siv nyob rau hauv fluorescence microscopy.
Ultra-siab siab mercury teeb kuj emit ntau cua sov. Yog li ntawd, lub teeb lub tsev yuav tsum muaj cov cua kub dissipation zoo, thiab qhov chaw ua haujlwm kub yuav tsum tsis txhob siab dhau.
Tus tshiab ultra-siab siab mercury teeb tuaj yeem ignited yam tsis muaj hluav taws xob siab thaum pib siv. Tom qab ib lub sij hawm ntawm kev siv, nws yuav tsum tau pib ntawm high voltage (li 15000V). Tom qab pib, kev saib xyuas ua haujlwm voltage feem ntau yog 50-60V, thiab qhov ua haujlwm tam sim no yog li 4A. Qhov nruab nrab lifespan ntawm 200W ultra-siab siab mercury teeb yog hais txog 200 teev yog tias nws siv rau 2 teev txhua zaus. Lub sijhawm ua haujlwm luv dua, lub neej luv dua. Yog tias nws tsuas yog siv 20 feeb xwb, lub neej yuav raug txo los ntawm 50 feem pua. Yog li ntawd, txo tus naj npawb ntawm cov pib thaum siv nws. Thaum lub sij hawm siv lub teeb, nws cov luminous efficiency yog maj txo. Tom qab lub teeb tawm, tos kom nws txias ua ntej rov pib dua. Tsis txhob tua lub teeb tam sim ntawd tom qab igniting nws, thiaj li tsis mus ua puas lub electrode vim tsis tiav mercury evaporation. Feem ntau, nws yuav tsum tau tos 15 feeb. Vim lub siab kub siab ntawm lub teeb hluav taws xob ultra-siab mercury thiab cov hluav taws xob muaj zog ultraviolet, lub teeb yuav tsum tau muab tso rau hauv lub teeb chamber ua ntej nws tuaj yeem ignited, thiaj li tsis ua rau lub qhov muag puas thiab ua haujlwm thaum muaj kev tawg. .
Lub voj voog ntawm lub teeb hluav taws xob ultra-siab mercury (100W lossis 200W) lub teeb ci thiab ntau qhov chaw suav nrog transformer, ballast thiab pib. Muaj ib qho kev kho qhov chaw luminous ntawm lub teeb nyob rau hauv lub teeb chamber. Ib qho txhuas-plated concave reflector yog ntsia tom qab lub teeb qhov muag teev, thiab lub teeb khaws lub teeb yog ntsia rau pem hauv ntej.
Lub domestic ultra-siab siab mercury teeb GCQ-200 muaj kev ua tau zoo thiab tuaj yeem hloov cov qij txawv teb xws li HBO-200. Qhov nruab nrab lifespan yog ntau tshaj 200h thiab tus nqi kuj tsawg.
Ib qho yooj yim thiab portable siab xim kub bromine tungsten fluorescent lub teeb qhov khoom siv tsim nyob rau hauv kuv lub teb chaws muaj me me, lub teeb yuag, tsis muaj zog, AC thiab DC dual-siv (nrog DC fais fab mov), yooj yim nqa, yooj yim siv, thiab muaj tau nrov thiab siv.
(2) Xim lim system
Cov xim lim dej yog ib feem tseem ceeb ntawm lub tshuab hluav taws xob fluorescence, uas muaj cov phaj lim dej thiab cov phaj lim nias. Cov qauv lim phaj thiab lub npe ntawm txhua tus neeg tsim khoom feem ntau tsis sib xws. Cov ntawv lim feem ntau muaj npe tom qab cov xim yooj yim, cov tsiaj ntawv nyob rau hauv pem hauv ntej sawv cev rau xim, cov tsiaj ntawv nyob tom qab sawv cev rau iav, thiab cov lej sawv cev rau cov yam ntxwv ntawm cov qauv. olympus microscope
(3) Lub hom phiaj lens
Ntau lub hom phiaj lo ntsiab muag tuaj yeem siv tau, tab sis achromatic lub hom phiaj lo ntsiab muag yog siv vim tias lawv cov autofluorescence tsawg thiab lawv lub teeb kis tau tus mob (nruab nrab wavelength) yog haum rau fluorescence. Txij li thaum lub fluorescence brightness ntawm daim duab nyob rau hauv lub microscope ntawm saib yog proportional rau lub square ntawm lub aperture piv ntawm lub hom phiaj lens thiab inversely proportional rau nws magnification, thiaj li yuav txhim kho lub brightness ntawm cov duab fluorescence, lub hom phiaj lens nrog ib tug loj. aperture ratio yuav tsum tau siv. Tshwj xeeb tshaj yog nyob rau hauv high magnification nws cov nyhuv yog pom tseeb heev. Yog li ntawd, rau cov qauv uas tsis muaj fluorescence tsis txaus, lub hom phiaj lens nrog qhov loj aperture piv yuav tsum tau siv nrog lub ntsej muag kom tsawg li sai tau (4 ×, 5 ×, 6.3 ×, thiab lwm yam).
(4) Daim iav
Cov txheej txheej ntawm cov reflector feem ntau yog txhuas-plated, vim tias txhuas absorbs tsawg dua nyob rau hauv lub xiav-violet cheeb tsam ntawm ultraviolet lub teeb thiab pom lub teeb, thiab lub reflection yog ntau tshaj li 90 feem pua, thaum lub reflection ntawm nyiaj tsuas yog 70 feem pua. Feem ntau, lub tiaj tus reflector yog siv.
(5) Condenser
Condensers tsim los rau fluorescence microscopy yog ua los ntawm quartz iav los yog lwm yam UV-pob tshab iav. Muaj ob hom clear field condensers thiab dark field condensers. Kuj tseem muaj cov theem sib txawv fluorescence concentrators.
(6) Epi-light device
Hom tshiab ntawm lub teeb pom kev zoo yog tias tom qab lub teeb los ntawm lub teeb pom kev cuam tshuam cov lim spectroscopic, qhov luv wavelength feem (ultraviolet thiab liab doog xiav) yog tshwm sim vim yog cov khoom ntawm txheej ntawm lub lim. Thaum lub lim tawm ntawm lub teeb pom kev, lub kaum sab xis yog 45. Thaum nws qaij, nws tua mus rau lub hom phiaj lens vertically, thiab tua rau cov qauv los ntawm lub hom phiaj lens, kom cov qauv zoo siab. Lub sijhawm no, lub hom phiaj lens ncaj qha ua raws li lub teeb khaws cia. Nyob rau tib lub sijhawm, qhov ntev ntawm lub lim (ntsuab, daj, liab, thiab lwm yam) yog pob tshab rau lub lim, yog li nws tsis cuam tshuam rau kev coj ntawm lub hom phiaj lens, thiab lub lim ua raws li lub siab lim phaj, vim hais tias cov fluorescence ntawm cov qauv yog nyob rau hauv cheeb tsam ntev-wavelength ntawm pom lub teeb, muaj peev xwm dhau los ntawm lub lim thiab ncav cuag lub eyepiece rau kev soj ntsuam, lub brightness ntawm cov duab fluorescent nce nrog qhov nce ntawm qhov magnification, thiab muaj zog tshaj lub teeb kis tau tus mob. qhov chaw ntawm high magnification. Ntxiv nrog rau kev ua haujlwm ntawm lub teeb xa hluav taws xob, nws yog qhov tsim nyog rau kev soj ntsuam ncaj qha ntawm cov opaque thiab translucent specimens, xws li cov tuab tuab, lim membranes, colonies, thiab cov ntaub so ntswg kab lis kev cai. Cov fluorescence microscopes tshiab tsim nyob rau xyoo tas los no feem ntau siv cov khoom siv hluav taws xob, uas yog hu ua epi-fluorescence microscopes.
