Tus qauv thiab cov khoom siv tseem ceeb ntawm lub tsom iav fluorescence
(1) lub teeb ci
Tam sim no, ib 200W ultra - kub merculy teeb tau siv ua lub teeb pom kev. Nws yog ua los ntawm quartz iav thiab muaj cov duab me ntsis hauv nruab nrab, muaj qee qhov ntau ntawm mercury. Thaum lub sijhawm ua haujlwm, tso tawm ntawm ob lub electrodes ua rau muaj kev sib tw ua kom muaj kev lom zem, thiab lub siab sab hauv lub teeb nrawm nrawm. Thaum cov mercury kiag li evaporates, nws tuaj yeem ncav cuag 50 -} tus qauv atmospheric nias, uas feem ntau siv sijhawm 5-15 feeb. Lub lumcescence ntawm ultra-siab siab mercury mercury yog qhov txiaj ntsig ntawm cov electrodes, uas tsis tu ncua cov molecules, emitting teeb photons. Nws emits muaj zog ultraviolet thiab xiav violet lub teeb, yog li ntawd, nws yog dav siv nyob rau hauv fluorescence mic fluoresces.
(2) Lub cev lim xim
Qhov kev lim dej xim yog qhov tseem ceeb ntawm lub tsom iav fluorescence, muaj cov phaj txaus siab thiab lim lim phaj. Lub lim phaj lim thiab cov khoom lag luam npe feem ntau tsis sib haum. Lim daim hlau feem ntau muaj npe tom qab cov tsiaj ntawv xim yooj yim, nrog cov tsiaj ntawv nyob rau hauv pem hauv ntej uas sawv cev xim suab nrov, thiab cov naj npawb sawv cev cov yam ntxwv. Olympus microscope
(3) lub hom phiaj lens
Txhua yam ntawm lub hom phiaj lo ntsiab muag tuaj yeem siv, tab sis Achorratic cov iav yog qhov tsim nyog rau fluorescence thiab lub teeb kis tus mob (wavelength ntau yam kev kawm (wavelength ntau yam kev kawm (wavelength ntau yam kev kawm (wavelength). Vim yog qhov tseeb tias lub teeb ci fluorescence ntawm cov duab hauv lub cim ci ci ntsa iab ntawm lub hom phiaj fluorection ntawm lub hom phiaj fluorection ntawm lub ntsiab lus zoo dua qub nrog kev siv apert kawg yuav tsum tau siv. Tshwj xeeb tshaj yog nyob rau kev magnification siab, nws qhov kev cuam tshuam yog qhov tseem ceeb heev. Yog li ntawd, rau cov hnoos qeev nrog cov fluorescence tsis txaus, ib qho kev sib xyaw ua ke nrog lub ntsej muag zoo li tsawg tau (4 ×, 6.3 ×, thiab lwm yam).
(4) tsom iav
Cov txheej txheem reflective ntawm daim iav yog feem ntau yog coated nrog txhuas thaj chaw, nrog tus nqi sib zog ntawm ntau dua 90%, thaum muaj nyiaj tsuas yog xav txog 70%; Feem ntau, tsom iav yog siv.
