STED thiab immersion microscopes nrog kev tsom mus tob
Lub hom phiaj sib sib zog nqus ntawm siab NA lub hom phiaj ua rau me me PSF (qhov taw qhia kis tau tus mob), uas yog qhov tseem ceeb rau kev daws teeb meem microscopy systems. Hauv ntau lwm lub tshuab tsom iav, xws li immersion microscopes, lub npog npog yog siv los cais cov kua immersion los ntawm cov qauv. Qhov no tuaj yeem cuam tshuam PSF ntawm lub dav hlau focal. Peb pom tau hais tias asymmetric PSF yog ntxiv elongated tom qab daim npog. Tsis tas li ntawd, STED (Stimulated Emission Depletion) microscopy, uas yog siv dav nrog kev daws teeb meem ntawm kaum ntawm nanometers, siv lub toroidal PSF. Ua raws li txoj hauv kev uas tau hais los ntawm P.Török thiab PRT Monro, peb ua qauv ntawm qhov tob tob ntawm Gauss-Raggler beam. Ua qauv qhia yuav ua li cas los tsim ib lub voj voog PSF.
Sib sib zog nqus tsom nrog High NA Immersion Microscopy
Hauv VirtualLab Fusion, qhov cuam tshuam ntawm cov npog npog ntawm PSF tuaj yeem txheeb xyuas ncaj qha. Focal distortion tom qab daim npog npog yog ua kom pom thiab soj ntsuam nyob rau hauv tag nrho vectorial zam.
Ua kom pom tseeb ntawm Gaussian-Laguerre kab teeb hauv STED microscope
Nws tau pom tias kev tsom mus rau kev txiav txim siab Gaussian-Laguerre beams ua rau lub nplhaib zoo li PSF. Qhov loj ntawm lub annular PSF nyob ntawm, ntawm lwm qhov sib txawv, ntawm qhov kev txiav txim tshwj xeeb ntawm cov nqaj.
