Zoo sib xws thiab sib txawv ntawm fluorescence microscopes thiab kho qhov muag me me
Fluorescence microscope uses ultraviolet light as a light source to irradiate the object being inspected, causing it to emit fluorescence, and then observe the shape and position of the object under the microscope. Fluorescence microscopy yog siv los kawm qhov nqus, thauj, faib khoom, thiab cov tshuaj hauv zos hauv cov tshuaj.
Qee cov tshuaj hauv cov qog, xws li chlorophyll, tuaj yeem fluoresce thaum raug rau ultraviolet hluav taws xob; Tseem muaj qee yam tshuaj uas tsis tuaj yeem tawm fluorescence lawv tus kheej, tab sis kuj tseem tuaj yeem tso cov tshuaj tsw qab lossis ua kom muaj cov tshuaj lom fluorescodies thiab irradiated nrog ultraviolet teeb. Fluorescence microscopy yog ib qho ntawm cov cuab yeej rau cov kev tshawb fawb thiab kev tshawb fawb kom pom tseeb ntawm cov tshuaj no.
Muaj cov kab sib txawv hauv qab no ntawm fluorescence microscope thiab zoo tib yam tsom:
1. Lub illumination txoj kev feem ntau yog hom kev poob, qhov chaw ua rau lub teeb ci yog npaj rau ntawm cov qauv los ntawm lub hom phiaj lens;
2. Lub teeb ci ci yog ultraviolet teeb, nrog tus luv nthwv yoj thiab kev daws teeb meem siab dua li cov tsom iav zoo tib yam;
3. Muaj ob lub taub lim dej tshwj xeeb, ib qho ntawm lub teeb ci rau lub teeb pom kev zoo thiab lub ntsiab ntawm lub teeb ci thiab lub hom phiaj siv los tiv thaiv tib neeg lub qhov muag.
Fluorescence tsom kuj tseem yog hom tsom tsom rau kho qhov muag, nrog qhov sib txawv tseem ceeb yog qhov kev xav zoo siab. Qhov no txiav txim siab qhov sib txawv ntawm cov qauv thiab kev siv cov txheej txheem ntawm fluorescence microscopes zoo tib yam.
Fuzenceescence tsom kwm yog kev siv peev txheej rau immunofluorescence cell chemical. Nws yog tsim ntawm cov khoom tseem ceeb xws li lub teeb pom kev, lim phaj kaw lus, thiab qhov system optical. Nws yog kev siv lub teeb ntawm ib qho wavelength kom ua rau muaj cov quav tawm los ntawm lub hom phiaj fluorescence, uas yog tom qab lub hom phiaj fluorescence thiab soj ntsuam cov tshuaj fluorescence ntawm cov quav fluorescen.
