Ob peb yav tom ntej kev loj hlob cov lus qhia ntawm kis tau tus mob electron microscopy
Kev xa tawm electron microscopy ua haujlwm ua kom nrawm thiab muaj zog electron beam mus rau ib qho qauv nyias heev. Cov electrons sib tsoo nrog atoms hauv cov qauv thiab hloov cov kev taw qhia, yog li tsim cov khoom tawg tawg. Qhov luaj li cas ntawm lub kaum sab xis yog cuam tshuam nrog qhov ntom thiab tuab ntawm cov qauv, yog li cov duab sib txawv ntawm lub teeb thiab tsaus xim tuaj yeem tsim. Cov duab yuav raug tso tawm ntawm cov khoom siv duab (xws li cov ntxaij vab tshaus fluorescent, cov yeeb yaj kiab, thiab cov khoom sib txuas ntawm cov duab) tom qab kev nthuav dav thiab tsom.
Tam sim no, kis tau tus mob electron microscopy muaj ob peb txoj kev txhim kho tseem ceeb. , kev daws teeb meem txhim kho. Kev daws teeb meem yeej ib txwm yog lub hom phiaj thiab kev coj ua ntawm kev txhim kho kev sib kis ntawm electron microscopes. Tsim ib tiam tshiab ntawm monochromators thiab spherical aberration correctors los txhim kho lub zog daws teeb meem thiab spatial daws teeb meem ntawm kis tau tus mob electron microscopes, tshwj xeeb tshaj yog rau low-voltage electron microscopes. Thib ob, txhim kho nyob rau hauv-situ kis tau tus mob electron microscopy technology. Nyob rau hauv-situ kis tau tus mob electron microscopy muaj cov ntaub ntawv tseem ceeb nyob rau hauv lub teb ntawm cov khoom synthesis, tshuaj catalysis, lub neej sciences thiab lub zog cov ntaub ntawv. Nws tuaj yeem soj ntsuam thiab tswj kev nce qib ntawm cov roj-theem cov tshuaj tiv thaiv thiab cov kua-theem cov tshuaj tiv thaiv hauv lub sijhawm tiag tiag ntawm atomic scale, yog li kawm txog cov teeb meem kev tshawb fawb xws li cov txheej txheem tseem ceeb ntawm cov tshuaj tiv thaiv. Thib peb, nws yog dav siv nyob rau hauv txoj kev tshawb no ntawm biological macromolecule lug. Kev siv dav dav ntawm cryo-electron microscopy hauv kev tshawb fawb ntawm cov qauv macromolecule lom yuav txhawb kev txhim kho txuas ntxiv ntawm cryo-electron microscopy technology. Daim ntawv thov ntawm cryo-electron microscopy nyob rau hauv lub teb ntawm biology tau nyiam ntau thiab ntau xim, dhau los ua ib qho kev sib txuas thiab txuas txuas ntawm cov macromolecules thiab cov hlwb.
Nrog rau kev txhim kho tas mus li thiab kev nce qib ntawm electron microscopy, kev daws teeb meem ntawm kev xa xov hluav taws xob microscopy tau mus txog qib sub-angstrom, thiab electron microscopy tau dhau los ua tus cwj pwm tsis tseem ceeb hauv kev tshawb fawb cov ntaub ntawv. Nyob rau hauv ntau tshaj yim caum xyoo txij li thaum yug los ntawm kis tau tus mob electron microscopy mus rau niaj hnub no, tib neeg tau daws ntau yam teeb meem kev tshawb fawb nrog kev pab los ntawm kis tau tus mob electron microscopy. Kev xa xov hluav taws xob microscopes tseem tab tom txhim kho thiab nce zuj zus, nrog kev ua haujlwm dav dav thiab txhim kho kev ua haujlwm.
