Kev xav tau rau kev tsim cov fluorescent microscope specimens
(1) iav swb
Lub thickness ntawm tus swb yuav tsum nyob nruab nrab ntawm 0.8 thiab 1.2mm. Yog hais tias tus swb yog tuab heev, ntawm ib sab, nws absorbs lub teeb ntau dua, thiab ntawm qhov tod tes, nws tsis tuaj yeem ua rau lub teeb pom kev zoo sib sau ua ke ntawm cov qauv. Cov swb yuav tsum du, sib xws hauv tuab, thiab tsis muaj qhov pom tseeb ntawm cov fluorescence. Qee zaum quartz iav slides xav tau.
(2) Npog iav swb
Lub thickness ntawm lub khob iav yog hais txog 0.17mm, uas yog du thiab huv si. Txhawm rau txhim kho excitation, cuam tshuam npog iav kuj siv tau. Qhov no yog ib qho tshwj xeeb uas tsim los npog iav nrog ob peb txheej ntawm cov khoom (xws li magnesium fluoride) uas muaj kev cuam tshuam sib txawv ntawm lub teeb sib txawv ntawm lub teeb. Nws tuaj yeem tso cov fluorescence kom dhau mus zoo, thaum qhov kev xav kom muaj zog tuaj yeem ua rau cov qauv zoo.
(3) Cov qauv
Cov ntaub so ntswg los yog lwm yam qauv yuav tsum tsis txhob tuab heev, vim hais tias lub teeb excitation feem ntau yog noj nyob rau hauv qis qis ntawm cov qauv, thaum lub sab sauv ncaj qha soj ntsuam los ntawm lub hom phiaj lens yog tsis txaus siab heev. Tsis tas li ntawd, cell overlap los yog impurity masking tuaj yeem cuam tshuam rau kev txiav txim.
(4) Sealing agent
Glycerin feem ntau yog siv los ua tus neeg sawv cev mounting, thiab nws yuav tsum tsis pub muaj fluorescence, tsis muaj xim thiab pob tshab. Lub ci ci ntawm fluorescence yog ci dua ntawm pH 8.5 ~ 9.5, thiab nws tsis yooj yim kom ploj mus sai. Yog li ntawd, ib qho sib npaug ntawm glycerol thiab 0.5mol/l carbonate buffer ntawm pH 9.0-9.5 feem ntau yog siv los ua cov khoom sib khi.
(5) Daim iav roj
Thaum soj ntsuam cov qauv siv qhov chaw tsaus nti fluorescence microscope thiab lub tsom iav roj, nws yuav tsum tau siv cov roj lo ntsiab muag. Nws yog qhov zoo tshaj plaws los siv cov roj tshwj xeeb uas tsis muaj fluorescent, uas tuaj yeem hloov nrog glycerol raws li tau hais los saum no. Cov kua paraffin kuj tseem siv tau, tab sis qhov ntsuas qhov ntsuas tsis zoo thiab muaj kev cuam tshuam me ntsis ntawm cov duab zoo.
Kev ceev faj rau kev siv fluorescence microscope
(1) Ua raws nraim li lub Hoobkas phau ntawv yuav tsum tau ntawm lub fluorescence microscope thiab tsis txhob hloov qhov kev pab cuam arbitrarily.
(2) Kev tshuaj xyuas yuav tsum tau ua hauv chav tsaus. Tom qab nkag mus rau hauv chav tsaus, txuas cov khoom siv hluav taws xob thiab teeb lub teeb ci ultra-siab mercury rau 5 mus rau 15 feeb. Tom qab lub teeb pom kev zoo emits lub teeb muaj zog thiab ruaj khov, lub qhov muag hloov mus rau qhov tsaus ntuj ua ntej pib soj ntsuam cov qauv.
(3) Txhawm rau tiv thaiv UV puas rau lub qhov muag, cov iav tiv thaiv yuav tsum tau hnav thaum kho lub teeb ci.
(4) Lub sijhawm tshuaj xyuas yuav tsum yog 1-2 teev txhua lub sijhawm. Yog tias nws dhau 90 feeb, qhov luminous siv ntawm ultra-siab siab mercury teeb maj mam txo qis thiab cov fluorescence tsis muaj zog; Tom qab raug hluav taws xob ultraviolet rau 3-5 feeb, cov fluorescence ntawm cov qauv kuj txo qis; Yog li, nws yuav tsum tsis txhob ntau tshaj 2-3 teev.
(5) Lub neej ntev ntawm fluorescent microscope lub teeb pom kev yog txwv, thiab cov qauv yuav tsum tau kuaj xyuas hauv nruab nrab kom txuag tau sijhawm thiab tiv thaiv lub teeb ci. Thaum huab cua kub, lub kiv cua hluav taws xob yuav tsum tau muab ntxiv kom dissipate cua sov thiab txias. Thaum hloov lub teeb tshiab, lub sijhawm siv yuav tsum tau sau tseg txij thaum pib. Thaum lub teeb raug kaw thiab xav siv dua, nws yuav tsum tau tos kom lub teeb kom txias tag ua ntej yuav taws. Tsis txhob igniting lub teeb ci ntau zaus.
(6) Saib xyuas cov qauv tam sim tom qab staining, vim tias cov fluorescence maj mam txo qis thaum lub sijhawm. Yog tias cov qauv khaws cia hauv lub hnab yas polyethylene ntawm 4 degree, nws tuaj yeem ncua lub sijhawm fluorescence attenuation thiab tiv thaiv cov evaporation ntawm tus neeg sawv cev mounting.
(7) Tus qauv txiav txim rau fluorescence brightness feem ntau muab faib ua plaub theem, xws li "ib" - tsis muaj lossis pom tsis muaj zog fluorescence. ntxiv - Tsuas yog pom meej fluorescence tuaj yeem pom. ntxiv ntxiv - Pom pom nrog ci fluorescence. ' plus plus plus ' - Pom tau thiab dazzling fluorescence.
