Lub hauv paus ntsiab lus ntawm kev ua haujlwm thiab kev siv ntawm kev sib kis electron microscopy
Transmission Electron Microscope (TEM) tuaj yeem soj ntsuam cov qauv zoo me dua {{{0}}}.2um uas tsis tuaj yeem pom meej hauv qab lub tshuab ntsuas qhov muag. Cov qauv no hu ua submicroscopic structures lossis ultrastructures. Txhawm rau pom cov qauv no kom meej, nws yog qhov yuav tsum tau xaiv lub teeb lub teeb luv luv los txhim kho qhov kev daws teeb meem ntawm lub tshuab tsom. Xyoo 1932, Ruska tau tsim lub tshuab hluav taws xob xa hluav taws xob siv lub teeb hluav taws xob ua lub teeb ci. Lub wavelength ntawm lub electron beam yog luv dua li pom thiab ultraviolet lub teeb, thiab lub wavelength ntawm lub electron beam yog inversely proportional rau lub square root ntawm lub voltage ntawm lub emitted electron beam, txhais tau hais tias lub siab dua voltage, lub wavelength luv dua. Tam sim no, qhov kev daws teeb meem ntawm TEM tuaj yeem ncav cuag 0.2nm.
Lub hauv paus ntsiab lus ntawm kev ua haujlwm ntawm lub tshuab hluav taws xob xa hluav taws xob yog tias lub tshuab hluav taws xob hluav taws xob tawm los ntawm rab phom hluav taws xob hla dhau los ntawm lub condenser raws li qhov pom ntawm daim iav lub cev hauv lub tshuab nqus tsev, thiab sib sau ua ke rau hauv ib qho ntse, ci, thiab teeb pom kev zoo ntawm lub teeb. condenser, uas yog irradiated ntawm tus qauv hauv cov qauv chamber; Cov kab hluav taws xob hla dhau ntawm cov qauv nqa cov ntaub ntawv hauv cov qauv ntawm cov qauv. Tus nqi ntawm cov khoom siv hluav taws xob dhau los ntawm qhov ntom ntom ntawm cov qauv yog tsawg dua, thaum cov khoom siv hluav taws xob dhau los ntawm qhov sib txawv yog ntau dua; Tom qab tsom thiab thawj qhov kev nthuav dav los ntawm lub hom phiaj lens, cov kab hluav taws xob hluav taws xob nkag mus rau hauv qis nruab nrab lub lens thiab thawj thiab thib ob tsom iav tsom rau kev ua kom pom kev zoo. Thaum kawg, daim duab loj ntawm electron yog npaj rau ntawm lub vijtsam fluorescent hauv chav soj ntsuam; Lub vijtsam fluorescent hloov cov duab hluav taws xob rau hauv cov duab teeb pom kev zoo rau cov neeg siv saib. Tshooj lus no yuav qhia txog cov qauv tseem ceeb thiab cov hauv paus ntsiab lus ntawm txhua qhov system cais.
Kev siv cov kis tau tus mob electron microscopy
Transmission electron microscopy yog siv dav hauv cov ntaub ntawv science thiab biology. Vim qhov yooj yim tawg lossis nqus ntawm cov khoom siv hluav taws xob los ntawm cov khoom, qhov kev nkag mus yog qhov tsawg, thiab qhov ntom, tuab, thiab lwm yam ntawm cov qauv tuaj yeem cuam tshuam rau qhov kawg imaging zoo. Yog li ntawd, thinner ultra-thin slices, feem ntau yog 50-100nm, yuav tsum tau npaj. Yog li thaum soj ntsuam nrog lub tshuab hluav taws xob xa hluav taws xob, cov qauv yuav tsum tau ua tiav thinly. Cov txheej txheem ntau zaus suav nrog: Ultra-nyias cov txheej txheem ib txwm muaj, thiab lwm yam rau cov qauv ua kua, nws feem ntau yog pom los ntawm dai tus tooj liab mesh mesh.
